A simple radioisotopic assay for nucleoside kinases employing alumina for separation of nucleosides and nucleotides

Academic Article

Abstract

  • A rapid, simple, and sensitive radiochemical assay for the measurement of purine or pyrimidine nucleoside kinases (EC 2.7.1.-) is described. The substrate (thymidine, deoxyuridine, deoxycytidine, deoxyguanosine, deoxyadenosine, uridine, cytidine, and adenosine) is separated from the product (the respective 5′-nucleotide) on neutral alumina columns which retain the nucleotides but not the nucleosides. The nucleotides are recovered by elution with 0.4 m sodium phosphate buffer, pH 7.6. © 1981.
  • Digital Object Identifier (doi)

    Author List

  • el Kouni MH; Cha S
  • Start Page

  • 67
  • End Page

  • 71
  • Volume

  • 111
  • Issue

  • 1