Measurement and correction of transverse chromatic offsets for multi-wavelength retinal microscopy in the living eye

Academic Article


  • A special challenge arises when pursuing multi-wavelength imaging of retinal tissue in vivo, because the eye's optics must be used as the main focusing elements, and they introduce significant chromatic dispersion. Here we present an image-based method to measure and correct for the eye's transverse chromatic aberrations rapidly, non-invasively, and with high precision. We validate the technique against hyperacute psychophysical performance and the standard chromatic human eye model. In vivo correction of chromatic dispersion will enable confocal multi-wavelength images of the living retina to be aligned, and allow targeted chromatic stimulation of the photoreceptor mosaic to be performed accurately with sub-cellular resolution. © 2012 Optical Society of America.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Harmening WM; Tiruveedhula P; Roorda A; Sincich LC
  • Start Page

  • 2066
  • End Page

  • 2077
  • Volume

  • 3
  • Issue

  • 9