Maternal azithromycin therapy for Ureaplasma intraamniotic infection delays preterm delivery and reduces fetal lung injury in a primate model.

Academic Article

Abstract

  • OBJECTIVE: We assessed the efficacy of a maternal multidose azithromycin (AZI) regimen, with and without antiinflammatory agents to delay preterm birth and to mitigate fetal lung injury associated with Ureaplasma parvum intraamniotic infection. STUDY DESIGN: Long-term catheterized rhesus monkeys (n = 16) received intraamniotic inoculation of U parvum (10(7) colony-forming U/mL, serovar 1). After contraction onset, rhesus monkeys received no treatment (n = 6); AZI (12.5 mg/kg, every 12 h, intravenous for 10 days; n = 5); or AZI plus dexamethasone and indomethacin (n = 5). Outcomes included amniotic fluid proinflammatory mediators, U parvum cultures and polymerase chain reaction, AZI pharmacokinetics, and the extent of fetal lung inflammation. RESULTS: Maternal AZI therapy eradicated U parvum intraamniotic infection from the amniotic fluid within 4 days. Placenta and fetal tissues were 90% culture negative at delivery. AZI therapy significantly delayed preterm delivery and prevented advanced fetal lung injury, although residual acute chorioamnionitis persisted. CONCLUSION: Specific maternal antibiotic therapy can eradicate U parvum from the amniotic fluid and key fetal organs, with subsequent prolongation of pregnancy, which provides a therapeutic window of opportunity to effectively reduce the severity of fetal lung injury.
  • Keywords

  • Animals, Anti-Bacterial Agents, Anti-Inflammatory Agents, Anti-Inflammatory Agents, Non-Steroidal, Azithromycin, Chorioamnionitis, Dexamethasone, Drug Therapy, Combination, Female, Fetal Diseases, Indomethacin, Lung Injury, Macaca mulatta, Polymerase Chain Reaction, Pregnancy, Premature Birth, Treatment Outcome, Ureaplasma, Ureaplasma Infections
  • Digital Object Identifier (doi)

    Author List

  • Grigsby PL; Novy MJ; Sadowsky DW; Morgan TK; Long M; Acosta E; Duffy LB; Waites KB
  • Start Page

  • 475.e1
  • End Page

  • 475.e14
  • Volume

  • 207
  • Issue

  • 6