Palmitate stimulates glucose transport in rat adipocytes by a mechanism involving translocation of the insulin sensitive glucose transporter (GLUT4).

Academic Article


  • In rat adipocytes, palmitate: a) increases basal 2-deoxyglucose transport 129 +/- 27% (p less than 0.02), b) decreases the insulin sensitive glucose transporter (GLUT4) in low density microsomes and increases GLUT4 in plasma membranes and c) increases the activity of the insulin receptor tyrosine kinase. Palmitate-stimulated glucose transport is not additive with the effect of insulin and is not inhibited by the protein kinase C inhibitors staurosporine and sphingosine. In rat muscle, palmitate: a) does not affect basal glucose transport in either the soleus or epitrochlearis and b) inhibits insulin-stimulated glucose transport by 28% (p less than 0.005) in soleus but not in epitrochlearis muscle. These studies demonstrate a potentially important differential role for fatty acids in the regulation of glucose transport in different insulin target tissues.
  • Keywords

  • NASA Discipline Cell Biology, Non-NASA Center, 3-O-Methylglucose, Adipose Tissue, Animals, Cells, Cultured, Deoxyglucose, Glucose, Insulin, Kinetics, Macromolecular Substances, Male, Methylglucosides, Monosaccharide Transport Proteins, Muscles, Palmitic Acid, Palmitic Acids, Phosphotyrosine, Rats, Rats, Inbred Strains, Receptor, Insulin, Tyrosine
  • Author List

  • Hardy RW; Ladenson JH; Henriksen EJ; Holloszy JO; McDonald JM
  • Start Page

  • 343
  • End Page

  • 349
  • Volume

  • 177
  • Issue

  • 1