Epidemiologic usefulness of spoligotyping for secondary typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110

Academic Article


  • Restriction fragment length polymorphism (RFLP) analysis of IS6110 is commonly used to DNA fingerprint Mycobacterium tuberculosis. However, low-copy (≤5) IS6110 M. tuberculosis strains are poorly differentiated, requiring secondary typing. When spoligotyping was used as the secondary method, only 13% of Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains had epidemiologic linkages to another patient, compared to 48% of those with high-copy strains clustered by IS6110 alone (P<0.01). Spoligotyping did not improve a population-based molecular epidemiologic study of recent TB transmission.
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    Digital Object Identifier (doi)

    Author List

  • Cronin WA; Golub JE; Magder LS; Baruch NG; Lathan MJ; Mukasa LN; Hooper N; Razeq JH; Mulcahy D; Benjamin WH
  • Start Page

  • 3709
  • End Page

  • 3711
  • Volume

  • 39
  • Issue

  • 10