Adenovirus myocarditis: retrospective diagnosis by gene amplification from formalin-fixed, paraffin-embedded tissues.

Academic Article

Abstract

  • Understanding the pathogenesis of viral myocarditis is linked to the availability of sensitive assays to detect viruses in clinical material. Recent advances in molecular techniques permit direct detection of viral-specific nucleic acid in tissue samples. This report describes a protocol for DNA extraction and amplification of adenovirus genome from formalin-fixed, paraffin-embedded human tissues that detects as little as 10 copies of viral genome in a background of 0.5 micrograms of human DNA. This sensitive assay permitted the examination of archived tissues to establish a retrospective diagnosis of adenoviral myocarditis in two pediatric patients.
  • Authors

    Published In

  • Human Pathology  Journal
  • Keywords

  • Adenovirus Infections, Human, Adenoviruses, Human, Child, Child, Preschool, DNA, Viral, Female, Humans, Male, Myocarditis, Polymerase Chain Reaction, Tissue Preservation
  • Author List

  • Lozinski GM; Davis GG; Krous HF; Billman GF; Shimizu H; Burns JC
  • Start Page

  • 831
  • End Page

  • 834
  • Volume

  • 25
  • Issue

  • 8