Interleukins and IgA synthesis. Human and murine interleukin 6 induce high rate IgA secretion in IgA-committed B cells

Academic Article

Abstract

  • Freshly isolated murine PP B cells were cultured 10 different cytokines, including IL-1α, IL-2, IL-4, IL-5, IL-6, IL-7, IFN-γ, TNF-α, and TGF-β, to investigate a possible role for these cytokines in inductionof Ig synthesis. Of interest was the finding that only IL-5 and both mouse recombinant (mr) and human recombinant (hr) IL-6 enhanced IgA synthesis. The effect was greater with either mrIL-6 or hrIL-6 than with mrIL-5. IL-6 induced cycling mIgA + PP B cells to secrete high levels of IgA (~7-fold increase over control). Of importance was the finding that mrIL-6 had little effect on secretion of IgM or IgG by PP B cell cultures. hrIL-6 also increased IgA secretion by PP B cells and this enhancement was abolished by a goat anti-hr-IL-6 antiserum. mrIL-6 did not cause B cell proliferation but induced a sharp increase in numbers of B cells secreting IgA. Isotype-switching was not a mechanism for this marked increase in IgA synthesis since mIgA - PP B cells were not induced to secrete IgA by mrIL-6. From these studies we conclude that IL-6 plays an important role in promoting the terminal differentiation of PP B cells to IgA-secreting plasma cells.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 18768590
  • Author List

  • Beagley KW; Eldridge JH; Lee F; Kiyono H; Everson MP; Koopman WJ; Hirano T; Kishimoto T; McGhee J
  • Start Page

  • 2133
  • End Page

  • 2148
  • Volume

  • 169
  • Issue

  • 6