Column chromatography of antibodies and fragments.

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  • Since both size-exclusion (SE) chromatography (also known as gel filtration) and ion-exchange (IEX) chromatography are the fundamental methods on which antibody and fragment purifications are based, they are dealt with in detail in this appendix. The procedures for preparing the column matrix, for pouring the chromatography column, and for estimating the appropriate size of the column for the amount of antibody and fragment that are to be purified are all described.
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  • Smith JA
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  • Appendix 3