Cilofungin, a lipopeptide antifungal agent, was tested for in vitro activity alone and in combination with ketoconazole, itraconazole, flucytosine and amphotericin B against 102 clinical isolates of Candida species. At 48 hours all isolates of Candida albicans, Candida tropicalis, Candida paratropicalis and Candida glabrata were inhibited by ≤5 mcg/ml of cilofungin. In contrast, the MIC90 for Candida krusei was 10 mcg/ml and for Candida parapsilosis >40 mcg/ml. The interaction of combinations of cilofungin with amphotericin B, itraconazole, ketoconazole and flucytosine was additive or indifferent at 48 hours for 100 %, 88 %, 78 % and 70 % of all Candida species isolates, respectively. Overall, cilofungin demonstrated good activity in vitro against most Candida species isolates. © 1991 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.