Growth properties and alloantigenic expression of murine lymphoblastoid cell lines

Academic Article

Abstract

  • Murine lymphoblastoid cell lines were evaluated for their expression of Thy 1 and thymus leukemia (TL) differentiation alloantigens. Two culture conditions were shown to affect this expression. Cells grown in fetal bovine serum (FBS) enriched medium expressed up to 15 times the amount of TL as cells grown in horse serum (HS) enriched medium. Thy 1 expression was less affected by the type of serum used for culture. The phase of growth when the cells were harvested, was demonstrated to affect the expression of Thy 1. The expression of Thy 1.2 for one cell line examined, L 251A, during logarithmic growth was threefold greater than cells collected during either lag or stationary growth. When culture conditions were standardized a ranking of the amount of Thy 1 and TL expressed by several cell lines was made. All cell lines, except one, L 1210, expressed Thy 1. There was a 450 fold difference in the expression of Thy 1 between the cell lines evaluated. Seven cell lines expressed TL 1,2,3 with a ninefold difference in the amount of expression. The L 251A cell line was cultured in a 14 liter fermentor for a 26 day period. During this time TL and Thy 1 expression did not vary significantly, demonstrating that lymphoblastoid cell lines can be cultured on a continuous basis and will continue to express their surface alloantigens.
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    Author List

  • Zwerner RK; Acton RT
  • Start Page

  • 378
  • End Page

  • 390
  • Volume

  • 142
  • Issue

  • 2