Infiltration of CD4+ T cells into cornea during development of onchocerca volvulus-induced experimental sclerosing keratitis in mice

Academic Article


  • Sclerosing keratitis is the major cause of blindness due to onchocerciasis caused by the parasite Onchocerca volvulus. Although the importance of T cells in the pathogenesis of onchocerciasis has been suggested, their precise role in onchocercal sclerosing keratitis has not yet been defined. Using immunohistological techniques and a murine model of onchocercal sclerosing keratitis, we have performed a temporal analysis of the inflammatory T cells infiltrating into the cornea at days 4, 7, and 21 following intrastromal challenge with soluble O. volvulus antigens into presensitized mice. The maximum number of CD3+ T cells were observed in the corneal stroma at day 21 when sclerosing keratitis was most severe. The majority (>85%) of the CD3+ T cells were CD4+ at all time points. A few infiltrating cells bore IL-2 receptors indicating possible activation of a small fraction of the T cells. These results suggest that CD4+ T cells play an important role in onchocercal sclerosing keratitis. © 1994 Academic Press Inc.
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    Author List

  • Chakravarti B; Herring TA; Lass JH; Parker JS; Bucy RP; Diaconu E; Tseng J; Whitfield DR; Greene BM; Chakravarti DN
  • Start Page

  • 306
  • End Page

  • 314
  • Volume

  • 159
  • Issue

  • 2