Crystallization and preliminary X-ray analysis of the periplasmic receptor (PotF) of the putrescine transport system in Escherichia coli

Academic Article

Abstract

  • The primary receptor (PotF) of the putrescine transport system in E. coli has been crystallized by the hanging-drop vapordiffusion technique. The crystals belong to the space group P21212 with unit-cell dimensions a = 269.4, b = 82.33 and c = 93.74 Å. The crystals diffract beyond 2.2 Å with a rotatinganode X-ray source. A complete data set from the native crystals has been collected and processed at 2.3 Å resolution. Two heavy-atom derivatives have been prepared from the same Pt compound at 293 and 277 K. The difference Patterson maps revealed completely different major heavy-atom sites between these two derivatives. © 1998 International Union of Crystallography. All rights reserved.
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    Author List

  • Vassylyev DG; Kashiwagi T; Tomitori H; Kashiwagi K; Igarashi K; Morikawa K
  • Start Page

  • 132
  • End Page

  • 134
  • Volume

  • 54
  • Issue

  • 1