Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription factor DksA from Escherichia coli

Academic Article

Abstract

  • The Escherichia coli gene encoding a regulator of stringent response and virulence, DksA, which contains a canonical Zn finger motif, was cloned and expressed, and the purified protein was crystallized by the hanging-drop vapox-diffusion technique in two different space groups, P212 121 (a = 91.32, b = 96.59, c = 117.48 Å) and C222 (a = 80.6, b = 115.1, c = 149.57 Å). The crystals belonging to space group P212121, improved by macroseeding, diffract beyond 2.0 Å at a synchrotron. Three complete atomic resolution multiple anomalous dispersion diffraction data sets were collected from the same crystal of the P212121 crystal form at the absorption edge for Zn atoms. © 2004 International Union of Crystallography.
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    Author List

  • Vassylyeva MN; Perederina AA; Svetlov V; Yokoyama S; Artsimovitch I; Vassylyev DG
  • Start Page

  • 1611
  • End Page

  • 1613
  • Volume

  • 60
  • Issue

  • 9