Proton nuclear magnetic resonance measurement of the amide hydrogen exchange rates of group A streptococcal polysaccharide in H2O

Academic Article

Abstract

  • The solvent exchange rates of the acetamido hydrogen of the 2-acetamido-2-deoxy-β-D-glucopyranosyl unit of group A streptococcal polysaccharide dissolved in H2O have been measured and compared with the corresponding exchange rates in the solvated model compound 1-O-methyl-2-acetamido-2-deoxy-β-D-glucopyranoside. Amide hydrogen exchange rates were measured at 25°C over a wide pH range by a combination of two separate NMR techniques: (i) the transfer of solvent saturation and (ii) the amide hydrogen saturation recovery NMR experiments. The data indicate that the acetamido hydrogen essentially exists in a solvated environment and that its contribution to the conformational stability of this polysaccharide through intramolecular hydrogen bonding is negligible. © 1987.
  • Digital Object Identifier (doi)

    Author List

  • Huang DH; Pritchard DG; Sakai TT; Krishna NR
  • Start Page

  • 685
  • End Page

  • 690
  • Volume

  • 143
  • Issue

  • 2