Bullard, Daniel

Bullard, Daniel
Professor / Associate Dean

Senior Scientist, Inflammation, Immunology and Immunotherapeutics , O'Neal Comprehensive Cancer Center
Associate Scientist (C), Cell Adhesion & Matrix Research Center , Pathology 1996 -
Professor (P), Genetics Research Division , Genetics 2008 -
Scientist (C), Comprehensive Diabetes Center , School of Medicine 2011 -
Professor (S), Cell, Developmental and Integrative Biology (CDIB) , Academic Joint Departments 2012 -
Senior Scientist (C), Nephrology Research & Training Center , General Clinical Research Center 2014 -
Senior Scientist (C), Comprehensive Arthritis, Musculoskeletal, Bone and Autoimmunity Center (CAMBAC) , School of Medicine 2015 -
Scientist (C), Comprehensive Center for Healthy Aging , School of Medicine 2015 -
Scientist (C), Multiple Sclerosis Center , Neurology 2016 -
Professor (S), Dept of Medical Education , School of Medicine 2017 -
Assistant Dean, Dept of Medical Education , School of Medicine 2017 -
Associate Dean, Graduate School , The University of Alabama at Birmingham 2017 -
Senior Scientist (C), O'Neal Comprehensive Cancer Center , School of Medicine 2019 -

Overview

Dr. Bullard received his undergraduate degree in zoology from Iowa State University and his Ph.D. in genetics from Case Western Reserve University. During his postdoctoral studies in the Department of Human and Molecular Genetics at Baylor College of Medicine in Houston, he initiated research investigating the role of leukocyte/endothelial cell adhesion molecules in inflammatory processes. He joined the Department of Genetics at UAB in 1996.

Selected Publications

Academic Article

Year	Title	Altmetric
2019	Mice expressing the variant rs1143679 allele of ITGAM (CD11b) show impaired DC-mediated T cell proliferation. Mammalian Genome. 2019
2016	The tumor-associated glycosyltransferase ST6Gal-I regulates stem cell transcription factors and confers a cancer stem cell phenotype. Current Protocols in Toxicology. 76:3978-3988. 2016
2015	P-selectin enhances growth and metastasis of mouse mammary tumors by promoting regulatory T cell infiltration into the tumors. Life Sciences. 131:11-18. 2015
2014	ICAM-1: Isoforms and phenotypes. Journal of Immunology. 192:4469-4474. 2014
2014	Expression of a single ICAM-1 isoform on T cells is sufficient for development of experimental autoimmune encephalomyelitis. European Journal of Immunology. 44:1194-1199. 2014
2013	Multiple lupus-associated ITGAM variants alter mac-1 functions on neutrophils. Cancer Genetics and Cytogenetics. 65:2907-2916. 2013
2013	Experimental cerebral malaria develops independently of endothelial expression of intercellular adhesion molecule-1 (ICAM-1). Journal of Biological Chemistry. 288:10962-10966. 2013
2013	ICAM-1-dependent tuning of memory CD8 T-cell responses following acute infection. Proceedings of the National Academy of Sciences. 110:1416-1421. 2013
2012	Endothelial nitric oxide synthase inhibits the development of autoimmune-mediated vasculitis in mice. Cancer Genetics and Cytogenetics. 64:4114-4124. 2012
2012	Deletion of the complement phagocytic receptors CR3 and CR4 does not alter susceptibility to experimental cerebral malaria. Parasite Immunology. 34:547-550. 2012
2012	SDF-1α (CXCL12) regulation of lateral mobility contributes to activation of LFA-1 adhesion. Life Science Alliance. 303. 2012
2012	Microbial and histopathologic considerations in the use of mouse models of inflammatory bowel diseases. Inflammatory Bowel Diseases. 18:1558-1565. 2012
2011	ST6Gal-I regulates macrophage apoptosis via α2-6 sialylation of the TNFR1 death receptor. Journal of Biological Chemistry. 286:39654-39662. 2011
2010	Differential ICAM-1 isoform expression regulates the development and progression of experimental autoimmune encephalomyelitis. Molecular Immunology. 47:1692-1700. 2010
2009	γδ T cells in EAE: Early trafficking events and cytokine requirements. European Journal of Immunology. 39:1516-1526. 2009
2009	JAK3 inhibition significantly attenuates psoriasiform skin inflammation in CD18 mutant PL/J mice. Journal of Immunology. 183:2183-2192. 2009
2009	LFA-1 is critical for regulatory T cell homeostasis and function. Molecular Immunology. 46:2424-2428. 2009
2009	Mice that exclusively express TLR4 on endothelial cells can efficiently clear a lethal systemic Gram-negative bacterial infection. Journal of Clinical Investigation. 119:1921-1930. 2009
2009	Functional role of CD11c+ monocytes in atherogenesis associated with hypercholesterolemia. Materials Today: Proceedings. 119:2708-2717. 2009
2009	Effector and suppressor roles for LFA-1 during the development of experimental autoimmune encephalomyelitis. Journal of Neuroimmunology. 206:22-27. 2009
2008	Deletion of both ICAM-1 and C3 enhances severity of experimental autoimmune encephalomyelitis compared to C3-deficient mice. Neuroscience Letters. 442:158-160. 2008
2008	The integrin Mac-1 (CR3) mediates internalization and directs Bacillus anthracis spores into professional phagocytes. Proceedings of the National Academy of Sciences. 105:1261-1266. 2008
2007	Intercellular adhesion molecule-1 expression is required on multiple cell types for the development of experimental autoimmune encephalomyelitis. Journal of Immunology. 178:851-857. 2007
2007	p150/95 (CD11c/CD18) expression is required for the development of experimental autoimmune encephalomyelitis. Omega. 170:2001-2008. 2007
2006	Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice. Journal of Immunology. 177:8748-8756. 2006
2006	Review of autoimmune (lupus-like) glomerulonephritis in murine models. Ultrastructural Pathology. 30:345-359. 2006
2006	Intracellular heterogeneity in adhesiveness of endothelium affects early steps in leukocyte adhesion. Life Science Alliance. 291. 2006
2006	Transforming growth factor-β induces development of the T H17 lineage. Nature. 441:231-234. 2006
2006	Cerebral leucocyte infiltration in lupus-prone MRL/MpJ-fas^lpr mice - Roles of intercellular adhesion molecule-1 and P-selectin. Mol. Biol. Cell. 144:299-308. 2006
2005	Critical requirement of CD11b (Mac-1) on T cells and accessory cells for development of experimental autoimmune encephalomyelitis. Journal of Immunology. 175:6327-6333. 2005
2005	Aggravated lyme carditis in CD11a^-/- and CD11c^-/- mice. Infection and Immunity. 73:7637-7643. 2005
2005	PSGL-1 is not required for development of experimental autoimmune encephalomyelitis. Journal of Neuroimmunology. 166:193-196. 2005
2005	Accelerated development of arthritis in mice lacking endothelial selectins.. Microsurgery. 7. 2005
2005	Both Th1 and Th2 cells require P-selectin glycoprotein ligand-1 for optimal rolling on inflamed endothelium. Omega. 167:1661-1675. 2005
2005	P-selectin can support both Th1 and Th2 lymphocyte rolling in the intestinal microvasculature. Omega. 167:1647-1660. 2005
2004	The virulence function of Streptococcus pneumoniae surface protein A involves inhibition of complement activation and impairment of complement receptor-mediated protection. Journal of Immunology. 173:7506-7512. 2004
2004	Joint tests for quantitative trait loci in experimental crosses. Genetics Selection Evolution. 36:601-619. 2004
2004	Regulation of endothelial glutathione by ICAM-1: Implications for inflammation. Federation proceedings. 18:1321-1323. 2004
2004	Intercellular Adhesion Molecule-1 (ICAM-1) Regulates Endothelial Cell Motility through a Nitric Oxide-dependent Pathway. Journal of Biological Chemistry. 279:19230-19238. 2004
2004	Development of dermatitis in CD18-deficient PL/J mice is not dependent on bacterial flora, and requires both CD4⁺ and CD8⁺ T lymphocytes. International Immunology. 16:345-351. 2004
2004	Trafficking of Th1 Cells to Lung: A Role for Selectins and a P-Selectin Glycoprotein-1-Independent Ligand. International Journal for Numerical Methods in Engineering. 30:220-227. 2004
2004	Could labeled neuronal progenitor cells be used to target glioma tumor endothelium?. Current Opinion in Pulmonary Medicine. 3:845-846. 2004
2004	Loss of LFA-1, but not Mac-1, protects MRL/MpJ-Fas^lpr mice from autoimmune disease. Omega. 165:609-616. 2004
2003	High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium. Life Science Alliance. 285. 2003
2003	High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium.. Life Science Alliance. 285:C112-C118. 2003
2003	Psoriasiform dermatitis susceptibility in Itgb2^tm1Bay PL/J mice requires low-level CD18 expression and at least two additional loci for progression to severe disease. Omega. 163:197-202. 2003
2003	Preferential accumulation of antigen-specific effector CD4 T cells at an antigen injection site involves CD62E-dependent migration but not local proliferation. Journal of Experimental Medicine. 197:751-762. 2003
2003	Avidity Modulation Activates Adhesion under Flow and Requires Cooperativity among Adhesion Receptors. Journal of Human Lactation. 85:4122-4133. 2003
2003	Critical role of the α4 integrin/VCAM-1 pathway in cerebral leukocyte trafficking in lupus-prone MRL/fas^lpr mice. Journal of Immunology. 170:520-527. 2003
2002	Cutting-edge technology IV. Genomic engineering for studies of the gastrointestinal tract in mice. Maryland state medical journal. 283. 2002
2002	Cutting-edge technology: IV. Genomic engineering for studies of the gastrointestinal tract in mice.. Maryland state medical journal. 283:G1232-G1237. 2002
2002	Adhesion molecules in inflammatory diseases: Insights from knockout mice. Immunologic Research. 26:27-33. 2002
2002	Subset-specific reductions in lung lymphocyte accumulation following intratracheal antigen challenge in endothelial selectin-deficient mice. Journal of Immunology. 169:2570-2579. 2002
2002	A functional role for circulating mouse L-selectin in regulating leukocyte/endothelial cell interactions in vivo. Journal of Immunology. 169:2034-2043. 2002
2002	E- and P-selectins are essential for the development of cockroach allergen-induced airway responses. Journal of Immunology. 169:2120-2125. 2002
2002	Leukocyte-endothelial cell interactions are enhanced in dermal postcapillary venules of MRL/fas^lpr (lupus-prone) mice: Roles of P- and E-selectin. Journal of Immunology. 168:4728-4736. 2002
2002	Mice genetically lacking endothelial selectins are resistant to the lethality in septic peritonitis. Experimental and Molecular Pathology. 72:68-76. 2002
2001	Mind the phenotype gap. Molecular Medicine Today. 7:537-538. 2001
2001	Essential role of ICAM-1 in mediating monocyte adhesion to aortic endothelial cells. Life Science Alliance. 281. 2001
2001	Endothelial transcytosis of myeloperoxidase confers specificity to vascular ECM proteins as targets of tyrosine nitration. Journal of Clinical Investigation. 108:1759-1770. 2001
2001	Endothelial transcytosis of myeloperoxidase confers specificity to vascular ECM proteins as targets of tyrosine nitration.. Journal of Clinical Investigation. 108:1759-1770. 2001
2001	The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. European Journal of Immunology. 31:3085-3093. 2001
2001	Essential role of ICAM-1 in mediating monocyte adhesion to aortic endothelial cells.. Life Science Alliance. 281:C1442-C1447. 2001
2001	In vitro culture and characterization of gene targeted mouse endothelium. Clinical Microbiology Newsletter. 173:151-157. 2001
2001	Dermal and pulmonary inflammatory disease in E-selectin and P-selectin double-null mice is reduced in triple-selectin-null mice. Journal of Health Psychology. 98:727-735. 2001
2000	Leukocyte and endothelial cell adhesion molecules in a chronic murine model of myocardial reperfusion injury. Journal of Mid-Life Health. 279. 2000
2000	Extreme shear rate dependence of leukocyte rolling in CD18-/-E-selectin/-double mutant mice. JIMD Reports. 28. 2000
2000	Severe inflammatory defect and reduced viability in CD18 and E-selectin double-mutant mice. Journal of Clinical Investigation. 106:1457-1466. 2000
1999	Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration. Journal of Immunology. 163:5029-5038. 1999
1999	Acceleration and increased severity of collagen-induced arthritis in P- selectin mutant mice. Journal of Immunology. 163:2844-2849. 1999
1999	Roles of leukocyte/endothelial cell adhesion molecules in the pathogenesis of vasculitis. Electronic Journal of Biotechnology. 106:677-687. 1999
1999	Chronic inflammatory disease alters adhesion molecule requirements for acute neutrophil emigration in mouse skin. Journal of Immunology. 162:5444-5448. 1999
1998	Importance of E-selectin for firm leukocyte adhesion in vivo. Biologie Aujourd'hui. 83:287-294. 1998
1998	Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion. Journal of Mid-Life Health. 274. 1998
1998	Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion. Journal of Mid-Life Health. 43. 1998
1998	Combinatorial requirements for adhesion molecules in mediating neutrophil emigration during bacterial peritonitis in mice. Journal of Leukocyte Biology. 64:291-297. 1998
1997	L-selectin dependent leukocyte rolling in E-selectin and P-selectin double mutant mice. Federation proceedings. 11. 1997
1997	Host defense against systemic infection with Streptococcus pneumoniae is impaired in E-, P-, and E-/P-selectin-deficient mice. Journal of Clinical Investigation. 100:2099-2106. 1997
1997	Intercellular Adhesion Molecule-1 Deficiency Protects MRL/MpJ-Fas^lpr Mice from Early Lethality. Journal of Immunology. 159:2058-2067. 1997
1997	A minimal role for selectins in the recruitment of leukocytes into the inflamed liver microvasculature. Journal of Clinical Investigation. 99:2782-2790. 1997
1997	The association between α4-integrin, P-selectin, and E-selectin in an allergic model of inflammation. Journal of Experimental Medicine. 185:1077-1087. 1997
1997	LFA-1 is sufficient in mediating neutrophil emigration in Mac-1- deficient mice. Journal of Clinical Investigation. 99:1340-1350. 1997
1997	Deficiency of inflammatory cell adhesion molecules protects against atherosclerosis in mice. WSEAS Transactions on Communications. 17:1517-1520. 1997
1996	Defects in neutrophil function and host defense in IAP-deficient mice. Federation proceedings. 10. 1996
1996	LFA-1 is sufficient in mediating neutrophil transmigration in Mac-1 knockout mice. Federation proceedings. 10. 1996
1996	Velocity differences between L- and P-selectin-dependent neutrophil rolling in venules of mouse cremaster muscle in vivo. Journal of Mid-Life Health. 271. 1996
1996	The Role of P-Selectin and ICAM-1 in Acute Lung Injury as Determined Using Blocking Antibodies and Mutant Mice. Journal of Immunology. 157:4609-4614. 1996
1996	Decreased resistance to bacterial infection and granulocyte defects in IAP-deficient mice. Science. 274:795-798. 1996
1996	Inhibition of delayed-type contact hypersensitivity in mice deficient in both E-selectin and P-selectin. Journal of Health Psychology. 88:2973-2979. 1996
1996	Reduced Susceptibility to Collagen-Induced Arthritis in Mice Deficient in Intercellular Adhesion Molecule-1. Journal of Immunology. 157:3153-3158. 1996
1996	Selectins and neutrophil traffic: Margination and Streptococcus pneumoniae-induced emigration in murine lungs. Journal of Experimental Medicine. 184:639-645. 1996
1996	Infectious susceptibility and severe deficiency of leukocyte rolling and recruitment in E-selectin and P-selectin double mutant mice. Journal of Experimental Medicine. 183:2329-2336. 1996
1996	A polygenic mouse model of psoriasiform skin disease in CD18-deficient mice. Proceedings of the National Academy of Sciences. 93:2116-2121. 1996
1996	Absence of trauma-induced leukocyte rolling in mice deficient in both P-selectin and intercellular adhesion molecule 1. Journal of Experimental Medicine. 183:57-65. 1996
1995	Sequential contribution of l-and p-selectin to leukocyte rolling in vivo. Journal of Experimental Medicine. 181:669-675. 1995
1995	Gene targeting for inflammatory cell adhesion molecules. Annals of Oncology. 47:143-154. 1995
1995	P-selectin/ICAM-1 double mutant mice: Acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli. Journal of Clinical Investigation. 95:1782-1788. 1995
1992	Functional analysis of a t complex responder locus transgene in mice. Mammalian Genome. 3:579-587. 1992
1991	Molecular structure of Tcp-10 genes from the t complex responder locus. Mammalian Genome. 1:228-234. 1991
1990	Molecular cloning and genetic mapping of the t complex responder candidate gene family. Genetics. 124:957-966. 1990
1990	Molecular cloning of the t complex responder genetic locus. Genomics. 8:134-140. 1990

Chapter

Year	Title	Altmetric
2018	CR3. 435-450. 2018
2018	CR4. 451-461. 2018

Research Overview

Dr. Bullard’s research interests are centered on defining the mechanisms that regulate inflammation. For these investigations, we have focused our studies on defining the roles of leukocyte/endothelial cell adhesion molecules in mediating inflammatory responses. These proteins, along with chemoattractant/activating molecules, mediate the process by which leukocytes exit the vasculature into tissue in response to an inflammatory stimulus. Many different adhesion molecules have been described, including the selectins, integrins, and members of the immunoglobulin superfamily of adhesion receptors. Recent evidence from our lab and others suggest that these molecules play both pro- and anti-inflammatory roles, and we are using a genetic approach in mice to further define these functions. For our studies, we have developed many different lines of adhesion molecule mutant mice using gene-targeting methodologies. Mice with single or multiple mutations are currently being analyzed in models of rheumatoid arthritis, lupus erythematosus, vasculitis, and inflammatory bowel disease to determine their specific roles in the pathogenesis of these diseases. Our lab has also characterized a novel model for psoriasis and psoriatic arthritis that develops in Beta-2 integrin mutant mice backcrossed onto the PL/J strain background. We are now trying to map and clone the genes that control the development of both the skin disease and the arthritis in this model system.