Positions

Overview

  • Dr. Bullard received his undergraduate degree in zoology from Iowa State University and his Ph.D. in genetics from Case Western Reserve University. During his postdoctoral studies in the Department of Human and Molecular Genetics at Baylor College of Medicine in Houston, he initiated research investigating the role of leukocyte/endothelial cell adhesion molecules in inflammatory processes. He joined the Department of Genetics at UAB in 1996.

    • Selected Publications

    Academic Article

    Year Title Altmetric
    2016 The tumor-associated glycosyltransferase ST6Gal-I regulates stem cell transcription factors and confers a cancer stem cell phenotype.  Cancer Research.  76:3978-3988. 2016
    2015 P-selectin enhances growth and metastasis of mouse mammary tumors by promoting regulatory T cell infiltration into the tumors.  Life Sciences.  131:11-18. 2015
    2014 ICAM-1: Isoforms and phenotypes.  Journal of Immunology.  192:4469-4474. 2014
    2014 Expression of a single ICAM-1 isoform on T cells is sufficient for development of experimental autoimmune encephalomyelitis.  European Journal of Immunology.  44:1194-1199. 2014
    2013 Multiple lupus-associated ITGAM variants alter mac-1 functions on neutrophils.  Arthritis and Rheumatism.  65:2907-2916. 2013
    2013 Experimental cerebral malaria develops independently of endothelial expression of intercellular adhesion molecule-1 (ICAM-1).  Journal of Biological Chemistry.  288:10962-10966. 2013
    2013 ICAM-1-dependent tuning of memory CD8 T-cell responses following acute infection.  Proceedings of the National Academy of Sciences.  110:1416-1421. 2013
    2012 Endothelial nitric oxide synthase inhibits the development of autoimmune-mediated vasculitis in mice.  Arthritis and Rheumatism.  64:4114-4124. 2012
    2012 Deletion of the complement phagocytic receptors CR3 and CR4 does not alter susceptibility to experimental cerebral malaria.  Parasite Immunology.  34:547-550. 2012
    2012 SDF-1α (CXCL12) regulation of lateral mobility contributes to activation of LFA-1 adhesion.  American Journal of Physiology - Cell Physiology.  303. 2012
    2012 Microbial and histopathologic considerations in the use of mouse models of inflammatory bowel diseases.  Inflammatory Bowel Diseases.  18:1558-1565. 2012
    2011 ST6Gal-I regulates macrophage apoptosis via α2-6 sialylation of the TNFR1 death receptor.  Journal of Biological Chemistry.  286:39654-39662. 2011
    2010 Differential ICAM-1 isoform expression regulates the development and progression of experimental autoimmune encephalomyelitis.  Molecular Immunology.  47:1692-1700. 2010
    2009 γδ T cells in EAE: Early trafficking events and cytokine requirements.  European Journal of Immunology.  39:1516-1526. 2009
    2009 JAK3 inhibition significantly attenuates psoriasiform skin inflammation in CD18 mutant PL/J mice.  Journal of Immunology.  183:2183-2192. 2009
    2009 LFA-1 is critical for regulatory T cell homeostasis and function.  Molecular Immunology.  46:2424-2428. 2009
    2009 Mice that exclusively express TLR4 on endothelial cells can efficiently clear a lethal systemic Gram-negative bacterial infection.  Journal of Clinical Investigation.  119:1921-1930. 2009
    2009 Functional role of CD11c+ monocytes in atherogenesis associated with hypercholesterolemia.  Circulation.  119:2708-2717. 2009
    2009 Effector and suppressor roles for LFA-1 during the development of experimental autoimmune encephalomyelitis.  Journal of Neuroimmunology.  206:22-27. 2009
    2008 Deletion of both ICAM-1 and C3 enhances severity of experimental autoimmune encephalomyelitis compared to C3-deficient mice.  Neuroscience Letters.  442:158-160. 2008
    2008 The integrin Mac-1 (CR3) mediates internalization and directs Bacillus anthracis spores into professional phagocytes.  Proceedings of the National Academy of Sciences.  105:1261-1266. 2008
    2007 Intercellular adhesion molecule-1 expression is required on multiple cell types for the development of experimental autoimmune encephalomyelitis.  Journal of Immunology.  178:851-857. 2007
    2007 p150/95 (CD11c/CD18) expression is required for the development of experimental autoimmune encephalomyelitis.  American Journal of Pathology.  170:2001-2008. 2007
    2006 Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice.  Journal of Immunology.  177:8748-8756. 2006
    2006 Review of autoimmune (lupus-like) glomerulonephritis in murine models.  Ultrastructural Pathology.  30:345-359. 2006
    2006 Intracellular heterogeneity in adhesiveness of endothelium affects early steps in leukocyte adhesion.  American Journal of Physiology - Cell Physiology.  291. 2006
    2006 Transforming growth factor-β induces development of the T H17 lineage.  Nature.  441:231-234. 2006
    2006 Cerebral leucocyte infiltration in lupus-prone MRL/MpJ-faslpr mice - Roles of intercellular adhesion molecule-1 and P-selectin.  Clinical and Experimental Immunology.  144:299-308. 2006
    2005 Critical requirement of CD11b (Mac-1) on T cells and accessory cells for development of experimental autoimmune encephalomyelitis.  Journal of Immunology.  175:6327-6333. 2005
    2005 Aggravated lyme carditis in CD11a-/- and CD11c-/- mice.  Infection and Immunity.  73:7637-7643. 2005
    2005 PSGL-1 is not required for development of experimental autoimmune encephalomyelitis.  Journal of Neuroimmunology.  166:193-196. 2005
    2005 Accelerated development of arthritis in mice lacking endothelial selectins..  Arthritis Research.  7. 2005
    2005 Both Th1 and Th2 cells require P-selectin glycoprotein ligand-1 for optimal rolling on inflamed endothelium.  American Journal of Pathology.  167:1661-1675. 2005
    2005 P-selectin can support both Th1 and Th2 lymphocyte rolling in the intestinal microvasculature.  American Journal of Pathology.  167:1647-1660. 2005
    2004 The virulence function of Streptococcus pneumoniae surface protein A involves inhibition of complement activation and impairment of complement receptor-mediated protection.  Journal of Immunology.  173:7506-7512. 2004
    2004 Joint tests for quantitative trait loci in experimental crosses.  Genetics Selection Evolution.  36:601-619. 2004
    2004 Regulation of endothelial glutathione by ICAM-1: Implications for inflammation.  Federation proceedings.  18:1321-1323. 2004
    2004 Intercellular Adhesion Molecule-1 (ICAM-1) Regulates Endothelial Cell Motility through a Nitric Oxide-dependent Pathway.  Journal of Biological Chemistry.  279:19230-19238. 2004
    2004 Development of dermatitis in CD18-deficient PL/J mice is not dependent on bacterial flora, and requires both CD4+ and CD8+ T lymphocytes.  International Immunology.  16:345-351. 2004
    2004 Trafficking of Th1 Cells to Lung: A Role for Selectins and a P-Selectin Glycoprotein-1-Independent Ligand.  American Journal of Respiratory Cell and Molecular Biology.  30:220-227. 2004
    2004 Could labeled neuronal progenitor cells be used to target glioma tumor endothelium?.  Cancer Biology and Therapy.  3:845-846. 2004
    2004 Loss of LFA-1, but not Mac-1, protects MRL/MpJ-Faslpr mice from autoimmune disease.  American Journal of Pathology.  165:609-616. 2004
    2003 High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium.  American Journal of Physiology - Cell Physiology.  285. 2003
    2003 High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium..  American Journal of Physiology - Cell Physiology.  285:C112-C118. 2003
    2003 Psoriasiform dermatitis susceptibility in Itgb2tm1Bay PL/J mice requires low-level CD18 expression and at least two additional loci for progression to severe disease.  American Journal of Pathology.  163:197-202. 2003
    2003 Preferential accumulation of antigen-specific effector CD4 T cells at an antigen injection site involves CD62E-dependent migration but not local proliferation.  Journal of Experimental Medicine.  197:751-762. 2003
    2003 Avidity Modulation Activates Adhesion under Flow and Requires Cooperativity among Adhesion Receptors.  Biophysical Journal.  85:4122-4133. 2003
    2003 Critical role of the α4 integrin/VCAM-1 pathway in cerebral leukocyte trafficking in lupus-prone MRL/faslpr mice.  Journal of Immunology.  170:520-527. 2003
    2002 Cutting-edge technology IV. Genomic engineering for studies of the gastrointestinal tract in mice.  AJP - Gastrointestinal and Liver Physiology.  283. 2002
    2002 Cutting-edge technology: IV. Genomic engineering for studies of the gastrointestinal tract in mice..  AJP - Gastrointestinal and Liver Physiology.  283:G1232-G1237. 2002
    2002 Adhesion molecules in inflammatory diseases: Insights from knockout mice.  Immunologic Research.  26:27-33. 2002
    2002 Subset-specific reductions in lung lymphocyte accumulation following intratracheal antigen challenge in endothelial selectin-deficient mice.  Journal of Immunology.  169:2570-2579. 2002
    2002 A functional role for circulating mouse L-selectin in regulating leukocyte/endothelial cell interactions in vivo.  Journal of Immunology.  169:2034-2043. 2002
    2002 E- and P-selectins are essential for the development of cockroach allergen-induced airway responses.  Journal of Immunology.  169:2120-2125. 2002
    2002 Leukocyte-endothelial cell interactions are enhanced in dermal postcapillary venules of MRL/faslpr (lupus-prone) mice: Roles of P- and E-selectin.  Journal of Immunology.  168:4728-4736. 2002
    2002 Mice genetically lacking endothelial selectins are resistant to the lethality in septic peritonitis.  Experimental and Molecular Pathology.  72:68-76. 2002
    2001 Mind the phenotype gap.  Molecular Medicine Today.  7:537-538. 2001
    2001 Essential role of ICAM-1 in mediating monocyte adhesion to aortic endothelial cells.  American Journal of Physiology - Cell Physiology.  281. 2001
    2001 Endothelial transcytosis of myeloperoxidase confers specificity to vascular ECM proteins as targets of tyrosine nitration.  Journal of Clinical Investigation.  108:1759-1770. 2001
    2001 Endothelial transcytosis of myeloperoxidase confers specificity to vascular ECM proteins as targets of tyrosine nitration..  Journal of Clinical Investigation.  108:1759-1770. 2001
    2001 The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node.  European Journal of Immunology.  31:3085-3093. 2001
    2001 Essential role of ICAM-1 in mediating monocyte adhesion to aortic endothelial cells..  American Journal of Physiology - Cell Physiology.  281:C1442-C1447. 2001
    2001 In vitro culture and characterization of gene targeted mouse endothelium.  Acta Physiologica Scandinavica.  173:151-157. 2001
    2001 Dermal and pulmonary inflammatory disease in E-selectin and P-selectin double-null mice is reduced in triple-selectin-null mice.  Blood.  98:727-735. 2001
    2000 Leukocyte and endothelial cell adhesion molecules in a chronic murine model of myocardial reperfusion injury.  AJP - Heart and Circulatory Physiology.  279. 2000
    2000 Extreme shear rate dependence of leukocyte rolling in CD18-/-E-selectin/-double mutant mice.  Annals of Biomedical Engineering.  28. 2000
    2000 Severe inflammatory defect and reduced viability in CD18 and E-selectin double-mutant mice.  Journal of Clinical Investigation.  106:1457-1466. 2000
    1999 Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration.  Journal of Immunology.  163:5029-5038. 1999
    1999 Acceleration and increased severity of collagen-induced arthritis in P- selectin mutant mice.  Journal of Immunology.  163:2844-2849. 1999
    1999 Roles of leukocyte/endothelial cell adhesion molecules in the pathogenesis of vasculitis.  American Journal of Medicine.  106:677-687. 1999
    1999 Chronic inflammatory disease alters adhesion molecule requirements for acute neutrophil emigration in mouse skin.  Journal of Immunology.  162:5444-5448. 1999
    1998 Importance of E-selectin for firm leukocyte adhesion in vivo.  Circulation Research.  83:287-294. 1998
    1998 Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion.  AJP - Heart and Circulatory Physiology.  274. 1998
    1998 Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion.  AJP - Heart and Circulatory Physiology.  43. 1998
    1998 Combinatorial requirements for adhesion molecules in mediating neutrophil emigration during bacterial peritonitis in mice.  Journal of Leukocyte Biology.  64:291-297. 1998
    1997 L-selectin dependent leukocyte rolling in E-selectin and P-selectin double mutant mice.  Federation proceedings.  11. 1997
    1997 Host defense against systemic infection with Streptococcus pneumoniae is impaired in E-, P-, and E-/P-selectin-deficient mice.  Journal of Clinical Investigation.  100:2099-2106. 1997
    1997 Intercellular Adhesion Molecule-1 Deficiency Protects MRL/MpJ-Faslpr Mice from Early Lethality.  Journal of Immunology.  159:2058-2067. 1997
    1997 A minimal role for selectins in the recruitment of leukocytes into the inflamed liver microvasculature.  Journal of Clinical Investigation.  99:2782-2790. 1997
    1997 The association between α4-integrin, P-selectin, and E-selectin in an allergic model of inflammation.  Journal of Experimental Medicine.  185:1077-1087. 1997
    1997 LFA-1 is sufficient in mediating neutrophil emigration in Mac-1- deficient mice.  Journal of Clinical Investigation.  99:1340-1350. 1997
    1997 Deficiency of inflammatory cell adhesion molecules protects against atherosclerosis in mice.  Arteriosclerosis, Thrombosis, and Vascular Biology.  17:1517-1520. 1997
    1996 Defects in neutrophil function and host defense in IAP-deficient mice.  Federation proceedings.  10. 1996
    1996 LFA-1 is sufficient in mediating neutrophil transmigration in Mac-1 knockout mice.  Federation proceedings.  10. 1996
    1996 Velocity differences between L- and P-selectin-dependent neutrophil rolling in venules of mouse cremaster muscle in vivo.  AJP - Heart and Circulatory Physiology.  271. 1996
    1996 The Role of P-Selectin and ICAM-1 in Acute Lung Injury as Determined Using Blocking Antibodies and Mutant Mice.  Journal of Immunology.  157:4609-4614. 1996
    1996 Decreased resistance to bacterial infection and granulocyte defects in IAP-deficient mice.  Science.  274:795-798. 1996
    1996 Inhibition of delayed-type contact hypersensitivity in mice deficient in both E-selectin and P-selectin.  Blood.  88:2973-2979. 1996
    1996 Reduced Susceptibility to Collagen-Induced Arthritis in Mice Deficient in Intercellular Adhesion Molecule-1.  Journal of Immunology.  157:3153-3158. 1996
    1996 Selectins and neutrophil traffic: Margination and Streptococcus pneumoniae-induced emigration in murine lungs.  Journal of Experimental Medicine.  184:639-645. 1996
    1996 Infectious susceptibility and severe deficiency of leukocyte rolling and recruitment in E-selectin and P-selectin double mutant mice.  Journal of Experimental Medicine.  183:2329-2336. 1996
    1996 A polygenic mouse model of psoriasiform skin disease in CD18-deficient mice.  Proceedings of the National Academy of Sciences.  93:2116-2121. 1996
    1996 Absence of trauma-induced leukocyte rolling in mice deficient in both P-selectin and intercellular adhesion molecule 1.  Journal of Experimental Medicine.  183:57-65. 1996
    1995 Sequential contribution of l-and p-selectin to leukocyte rolling in vivo.  Journal of Experimental Medicine.  181:669-675. 1995
    1995 Gene targeting for inflammatory cell adhesion molecules.  Agents and Actions Supplements.  47:143-154. 1995
    1995 P-selectin/ICAM-1 double mutant mice: Acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli.  Journal of Clinical Investigation.  95:1782-1788. 1995
    1992 Functional analysis of a t complex responder locus transgene in mice.  Mammalian Genome.  3:579-587. 1992
    1991 Molecular structure of Tcp-10 genes from the t complex responder locus.  Mammalian Genome.  1:228-234. 1991
    1990 Molecular cloning and genetic mapping of the t complex responder candidate gene family.  Genetics.  124:957-966. 1990
    1990 Molecular cloning of the t complex responder genetic locus.  Genomics.  8:134-140. 1990

    Chapter

    Year Title Altmetric
    2017 CR3.  435-450. 2017
    2017 CR4.  451-461. 2017

    Research Overview

  • Dr. Bullard’s research interests are centered on defining the mechanisms that regulate inflammation. For these investigations, we have focused our studies on defining the roles of leukocyte/endothelial cell adhesion molecules in mediating inflammatory responses. These proteins, along with chemoattractant/activating molecules, mediate the process by which leukocytes exit the vasculature into tissue in response to an inflammatory stimulus. Many different adhesion molecules have been described, including the selectins, integrins, and members of the immunoglobulin superfamily of adhesion receptors. Recent evidence from our lab and others suggest that these molecules play both pro- and anti-inflammatory roles, and we are using a genetic approach in mice to further define these functions. For our studies, we have developed many different lines of adhesion molecule mutant mice using gene-targeting methodologies. Mice with single or multiple mutations are currently being analyzed in models of rheumatoid arthritis, lupus erythematosus, vasculitis, and inflammatory bowel disease to determine their specific roles in the pathogenesis of these diseases. Our lab has also characterized a novel model for psoriasis and psoriatic arthritis that develops in Beta-2 integrin mutant mice backcrossed onto the PL/J strain background. We are now trying to map and clone the genes that control the development of both the skin disease and the arthritis in this model system.

    • Principal Investigator On

    Investigator On

  • Summer Undergraduate Research Experiences in Genomic Medicine (SURE-GM)  awarded by National Human Genome Research Institute/NIH/DHHS 2018 - 2023
  • Effects of ITGAM Genetic Variation on Mac-1 Mediated Functions of B Cells  awarded by DOD - Department of Defense 2018 - 2021
  • Virus-Induced Cell Fate Decisions in Anti-Viral Immunity  awarded by National Institute of Allergy and Infectious Diseases/NIH/DHHS 2014 - 2019
  • Virus-Induced Cell Fate Decisions in Anti-Viral Immunity - Project 2 - Regulation of Anti-Viral CD8+ T Cell Responses Via Adhesion Molecules  awarded by National Institute of Allergy and Infectious Diseases/NIH/DHHS 2014 - 2019
  • Impact of SLE-Associated ITGAM Variants on Dendritic Cell Function  awarded by National Institute of Arthritis & Musculoskeletal & Skin Diseases/NIH/DHHS 2016 - 2019
  • Glycan Control of Stem Cell-Associated Pathways in Pancreatic Cancer  awarded by National Cancer Institute/NIH/DHHS 2015 - 2017
  • Glycosylation-Dependent Control of TNFR1 Signaling in Macrophage Survival  awarded by American Heart Association (Southeast Affiliate) 2014 - 2016
  • Training Program in Cardiovascular Pathophysiology  awarded by National Heart, Lung, and Blood Institute/NIH/DHHS 2014 - 2015
  • The Regulation of T Cell Exhaustion by Adhesion Molecules  awarded by National Institute of Allergy and Infectious Diseases/NIH/DHHS 2012 - 2015
  • UAB-UCSD O'Brien Core Center for Acute Kidney Injury Research  awarded by National Institute of Diabetes and Digestive and Kidney Diseases/NIH/DHHS 2008 - 2014

    • Education And Training

  • Doctor of Philosophy in Genetics, Case Western Reserve University 1992

    • Full Name

  • Daniel Bullard