Selected Publications

Academic Article

Year Title Altmetric
2019 Supercoil Levels in E. coli and Salmonella Chromosomes Are Regulated by the C-Terminal 35⁻38 Amino Acids of GyrA.Microorganisms.  7. 2019
2017 Efficient, ultra-high-Affinity chromatography in a one-step purification of complex proteinsProceedings of the National Academy of Sciences.  114:E5138-E5147. 2017
2016 Species-specific supercoil dynamics of the bacterial nucleoidBiophysical Reviews.  8:113-121. 2016
2015 Topological behavior of plasmid DNAMicrobiology Spectrum.  3. 2015
2014 RNA polymerase: Chromosome domain boundary maker and regulator of supercoil densityCurrent Opinion in Microbiology.  22:138-143. 2014
2012 A human TOP2A core DNA binding X-ray structure reveals topoisomerase subunit dynamics and a potential mechanism for SUMO modulation of decatenationJournal of Molecular Biology.  424:105-108. 2012
2012 Rates of Gyrase Supercoiling and Transcription Elongation Control Supercoil Density in a Bacterial ChromosomePLoS Genetics.  8. 2012
2010 DNA topology of highly transcribed operons in Salmonella enterica serovar TyphimuriumMicrobiological sciences.  78:1348-1364. 2010
2007 Microarray analysis of Mu transposition in Salmonella enterica, serovar Typhimurium: Transposon exclusion by high-density DNA binding proteinsMicrobiological sciences.  66:315-328. 2007
2007 Growth rate toxicity phenotypes and homeostatic supercoil control differentiate Escherichia coli from Salmonella enterica serovar typhimuriumJournal of Bacteriology.  189:5839-5849. 2007
2007 Mutational bias suggests that replication termination occurs near the dif site, not at Ter sites: What's the Dif?Microbiological sciences.  64:1-4. 2007
2005 A gyrase mutant with low activity disrupts supercoiling at the replication terminusJournal of Bacteriology.  187:7773-7783. 2005
2005 Organization of supercoil domains and their reorganization by transcriptionMicrobiological sciences.  57:1511-1521. 2005
2005 Bacteriophage Mu targets the trinucleotide sequence CGGJournal of Bacteriology.  187:3586-3588. 2005
2005 Measuring chromosome dynamics on different time scales using resolvases with varying half-livesMicrobiological sciences.  56:1049-1061. 2005
2004 Microarray analysis of transposition targets in Escherichia coli: The impact of transcriptionProceedings of the National Academy of Sciences.  101:9780-9785. 2004
2004 Transcription-induced barriers to supercoil diffusion in the Salmonella typhimurium chromosome.Proceedings of the National Academy of Sciences.  101:3398-3403. 2004
2004 Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts.Nucleic Acids Research.  32. 2004
2001 Mu and IS1 transpositions exhibit strong orientation bias at the Escherichia coli bgl locusJournal of Bacteriology.  183:3328-3335. 2001
2001 Transcription induces a supercoil domain barrier in bacteriophage MuBiochimie.  83:155-159. 2001
1999 Phage Mu transposition immunity reflects supercoil domain structure of the chromosomeMicrobiological sciences.  32:595-606. 1999
1998 Gyrase and Topo IV modulate chromosome domain size in vivoMicrobiological sciences.  29:1435-1448. 1998
1997 The DNA cleavage reaction of DNA gyrase. Comparison of stable ternary complexes formed with enoxacin and CcdB protein.Journal of Biological Chemistry.  272:27202-27209. 1997
1991 Topoisomerase mutants and physiological conditions control supercoiling and Z-DNA formation in vivo.Journal of Biological Chemistry.  266:2576-2581. 1991
1989 Supercoiling and integration host factor change the DNA conformation and alter the flow of convergent transcription in phage Mu.Journal of Biological Chemistry.  264:3035-3042. 1989
1988 Primary structure and mapping of the hupA gene of Salmonella typhimurium.Journal of Bacteriology.  170:5751-5758. 1988
1988 Variation of β-galactosidase expression from Mudlac elements during the development of Escherichia coli coloniesAnnales de l'Institut Pasteur. Microbiology.  139:79-103. 1988
1988 Variation of β-galactosidase expression from Mudlac elements during the development of Escherichia coli coloniesResearch in Microbiology.  139:79-103. 1988
1986 Positive and negative regulation of the Mu operator by Mu repressor and Escherichia coli integration host factorJournal of Biological Chemistry.  261:3744-3752. 1986
1986 Purification of the gam gene-product of bacteriophage mu and determination of the nucleotide sequence of the gam geneNucleic Acids Research.  14:6901-6914. 1986
1984 On the Mu repressor and early DNA intermediates of transpositionCold Spring Harbor Symposia on Quantitative Biology.  VOL. 49:827-834. 1984


Year Title Altmetric
2017 Measuring in vivo supercoil dynamics and transcription elongation rates in bacterial chromosomes.  17-27. 2017
2013 Gyrase.  374-377. 2013
2010 Molecular structure and dynamics of bacterial nucleoids.  117-148. 2010

Education And Training

  • Doctor of Philosophy in Microbiology, University of Chicago 1976
  • Full Name

  • N Higgins