Positions

Selected Publications

Academic Article

Year Title Altmetric
2016 Pillars Article: Identification of a Putative Second T-cell Receptor. Nature. 1986. 322: 145-149.Journal of Immunology.  196:3509-3513. 2016
2012 Engaging basic scientists in translational research: identifying opportunities, overcoming obstacles.Journal of Translational Medicine.  10:72. 2012
2010 Pathway modulators and inhibitorsCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]2010
2010 Analysis of proteins: IntroductionCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]2010
2010 Current Protocols in Molecular Biology: PrefaceCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]2010
2009 Pathway modulators and inhibitors.Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.].  Appendix 6:6. 2009
2008 Diagnosis of thrombotic thrombocytopenic purpura with normal ADAMTS13 activity and absence of its inhibitor (anti-ADAMTS13 antibodies)American Journal of Hematology.  83:256. 2008
2007 Performance of the Sebia CAPILLARYS 2 for detection and immunotyping of serum monoclonal paraproteinsAmerican Journal of Clinical Pathology.  128:293-299. 2007
2007 Bio*Medical informatics and genomic medicine: Research and trainingComputers and Biomedical Research.  40:1-4. 2007
2007 Electrospray ionisation-cleavable tandem nucleic acid mass tag-peptide nucleic acid conjugates: Synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MSNucleic Acids Research.  35. 2007
2006 Timing of plasma exchange and rituximab for the treatment of thrombotic thrombocytopenic purpura [1]American Journal of Clinical Pathology.  126:965. 2006
2006 Solution radioimmunoassay of proteins and peptides.Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.].  Chapter 10. 2006
2006 Spectrophotometric and colorimetric determination of protein concentration.Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.].  Chapter 10. 2006
2002 Electroelution of proteins from stained gels.Current protocols in immunology / edited by John E. Coligan ... [et al.].  Chapter 8. 2002
2001 Column chromatography of antibodies and fragments.Current protocols in immunology / edited by John E. Coligan ... [et al.].  Appendix 3. 2001
1999 Continuous quality improvement for point-of-care testing using background monitoring of duplicate specimensArchives of Pathology and Laboratory Medicine.  123:824-828. 1999
1999 Cost analysis for decision support: The case of comparing centralized versus distributed methods for blood gas testingJournal of Healthcare Management.  44:207-215. 1999
1999 Curbing overutilization: the silver lining to HCFA compliance.Medical Laboratory Observer.  31. 1999
1999 Estimating costs and turnaround times: presenting a user-friendly tool for analyzing costs and performance.Clinical Laboratory Management Review.  13:179-187. 1999
1998 Evaluating stat testing options in an academic health center: Therapeutic turnaround time and staff satisfactionClinical Chemistry.  44:1597-1603. 1998
1998 Stark choices for AHCsAcademic Medicine.  73:839-841. 1998
1997 A N(alpha)-acetyltransferase selectively transfers an acetyl group to NH2-terminal methionine residues: purification and partial characterization.BBA - Biochimica et Biophysica Acta.  1338:244-252. 1997
1997 A N(α)-acetyltransferase selectively transfers an acetyl group to NH2-terminal methionine residues: Purification and partial characterizationBBA - Proteins and Proteomics.  1338:244-252. 1997
1997 Training of doctors in the UK.Lancet.  349:436. 1997
1997 Using patient satisfaction as an indicator of the quality of laboratory services. Applying social science methods to evaluate outcomes in laboratory medicine.Clinical Laboratory Management Review.  11:93-102. 1997
1996 Acetyl-CoA hydrolase involved in acetate utilization in Saccharomyces cerevisiaeBBA - Proteins and Proteomics.  1297:105-109. 1996
1996 Automating clinical practice guidelines: A corporate-academic partnershipClinical Laboratory Management Review.  10:120-123. 1996
1993 Immunodominance: A single amino acid substitution within an antigenic site alters intramolecular selection of T cell determinantsJournal of Immunology.  151:1852-1858. 1993
1993 Isolation of a membrane-associated cathepsin D-like enzyme from the model antigen presenting cell, A20, and its ability to generate antigenic fragments from a protein antigen in a cell-free systemArchives of Biochemistry and Biophysics.  305:298-306. 1993
1993 Mapping of the glycoprotein 330 (gp330) gene to mouse chromosome 2Genomics.  17:780-781. 1993
1992 Immunodominance: Intermolecular competition between MHC class II molecules by covalently linked T cell epitopesJournal of Immunology.  148:3034-3041. 1992
1992 Molecular cloning, sequencing, deletion, and overexpression of a methionine aminopeptidase gene from Saccharomyces cerevisiaeJournal of Biological Chemistry.  267:8007-8011. 1992
1992 Silencing of immunodominant epitopes by contiguous sequences in complex synthetic peptidesCellular Immunology.  143:284-297. 1992
1991 Optimization of the Fragmentation in a Frit-Fast Atom Bombardment Ion Source for the Sequencing of Peptides at the Picomole LevelAnalytical Chemistry.  63:1978-1983. 1991
1991 Comparison of class I‐ and II‐restricted T cell recognition of the identical peptideEuropean Journal of Immunology.  21:2781-2789. 1991
1991 Immunodominance: Intramolecular competition between T cell epitopesJournal of Immunology.  146:2137-2144. 1991
1991 Primary Structure Determination of Peptides and Enzymatically Digested Proteins Using Capillary Liquid Chromatography/Mass Spectrometry and Rapid Linked-Scan TechniquesAnalytical Chemistry.  63:1091-1097. 1991
1991 Single amino acid substitution alters T cell determinant selection during antigen processing of Staphylococcus aureus nucleaseJournal of Immunology.  146:438-443. 1991
1990 Purification and characterization of a methionine aminopeptidase from Saccharomyces cerevisiaeJournal of Biological Chemistry.  265:19892-19897. 1990
1990 Model peptides reveal specificity of N(α)-acetyltransferase from Saccharomyces cerevisiaeJournal of Biological Chemistry.  265:11576-11580. 1990
1990 A glucose-repressible gene encodes acetyl-CoA hydrolase from Saccharomyces cerevisiaeJournal of Biological Chemistry.  265:7413-7418. 1990
1990 Identification of methionine N(α)-acetyltransferase from Saccharomyces cerevisiaeJournal of Biological Chemistry.  265:3603-3606. 1990
1990 Freshly isolated Thy-1+ dendritic epidermal cells express T cell receptor γδ-CD3Journal of Dermatological Science.  1:459-464. 1990
1990 Immunological activity of covalently linked T-cell epitopesNature.  343:381-383. 1990
1989 N alpha acetylation is required for normal growth and mating of Saccharomyces cerevisiae.Journal of Bacteriology.  171:5795-5802. 1989
1989 Nα-acetyltransferase deficiency alters protein synthesis in Saccharomyces cerevisiaeFEBS Letters.  256:139-142. 1989
1989 Structural organization of the rat acyl-peptide hydrolase gene.Nucleic Acids Research.  17:4397-4400. 1989
1989 Autoimmune target in Heymann nephritis is a glycoprotein with homology to the LDL receptorScience.  244:1163-1165. 1989
1989 Cloning and sequence analysis of a rat liver cDNA encoding acyl-peptide hydrolaseJournal of Biological Chemistry.  264:8892-8899. 1989
1989 High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codonsProceedings of the National Academy of Sciences.  86:1836-1840. 1989
1989 Identical peptides recognized by MHC class I- and II-restricted T cellsJournal of Experimental Medicine.  170:279-289. 1989
1989 Molecular cloning and sequencing of a cDNA encoding N(α)-acetyltransferase from Saccharomyces cerevisiaeJournal of Biological Chemistry.  264:12339-12343. 1989
1989 Molecular cloning and sequencing of genomic DNA encoding aminopeptidase I from Saccharomyces cerevisiaeJournal of Biological Chemistry.  264:6979-6983. 1989
1989 Murine MHC polymorphism and T cell specificitiesScience.  244:572-575. 1989
1989 N(α) acetylation is required for normal growth and mating of Saccharomyces cerevisiaeJournal of Bacteriology.  171:5795-5802. 1989
1989 Prediction of major histocompatibility complex binding regions of protein antigens by sequence pattern analysisProceedings of the National Academy of Sciences.  86:3296-3300. 1989
1989 Purification and characterization of an acetyl‐CoA hydrolase from Saccharomyces cerevisiaeEuropean Journal of Biochemistry.  184:21-28. 1989
1989 Relative contribution of 'determinant selection' and 'holes in the T-cell repertoire' to T-cell responsesProceedings of the National Academy of Sciences.  86:4649-4653. 1989
1988 Rapid separation of DNA restriction fragments using capillary electrophoresisChromatographic reviews.  458:323-333. 1988
1988 Structural characteristics of an antigen required for its interaction with Ia and recognition by T cellsNature.  328:395-399. 1988
1988 Identification of distinct C3B and C4B recognition sites in the human C3B/C4B receptor (Cri, Cd35) by deletion mutagenesisJournal of Experimental Medicine.  168:1699-1717. 1988
1988 T cell receptor gene usage in the response to γ repressor cI protein: An apparent bias in the usage of a V α gene elementJournal of Experimental Medicine.  168:1081-1097. 1988
1988 Organization of the genes encoding complement receptors type 1 and 2, decay-accelerating factor, and C4-binding protein in the RCA locus on human chromosome 1Journal of Experimental Medicine.  167:1271-1280. 1988
1988 Characterization of the catalytic domain of bovine adrenal tyrosine hydroxylaseBiochemical and Biophysical Research Communications.  151:1446-1453. 1988
1988 Structure of the human B lymphocyte receptor for C3d and the epstein-barr virus and relatedness to other members of the family of C3/C4 binding proteinsJournal of Experimental Medicine.  167:1047-1066. 1988
1988 Eosinophil granule cationic proteins: Major basic protein is distinct from the smaller subunit of eosinophil peroxidaseJournal of Leukocyte Biology.  43:1-4. 1988
1988 Eosinophilic Chemotactic Factors of AnaphylaxisMethods in Enzymology.  162:170-174. 1988
1988 Purification and characterization of an N alpha-acetyltransferase from Saccharomyces cerevisiae.Journal of Biological Chemistry.  263:14948-14955. 1988
1988 Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresisProceedings of the National Academy of Sciences.  85:9660-9663. 1988
1988 Sequence determination of the mumps virus HN geneVirology.  164:318-325. 1988
1987 T lymphocyte response to bacteriophage λ repressor cI protein. Recognition of the same peptide presented by Ia molecules of different haplotypesJournal of Immunology.  139:3973-3980. 1987
1987 Acyl-peptide hydrolase from rat liver. Characterization of enzyme reaction.Journal of Biological Chemistry.  262:11435-11445. 1987
1987 Human C3B/C4B receptor (CR1): Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristic of C3/C4 binding proteinsJournal of Experimental Medicine.  165:1095-1112. 1987
1987 A rat histone H4 gene closely associated with the testis-specific H1t geneExperimental Cell Research.  173:534-545. 1987
1987 Complete Amino Acid Sequences of the Heavy and Light Chain Variable Regions from Two A/J Mouse Antigen Nonbinding Monoclonal Antibodies Bearing the Predominant p-Azophenyl Arsonate IdiotypeBiochemistry.  26:604-612. 1987
1987 High-Performance Capillary Electrophoretic Separation of Bases, Nucleosides, and Oligonucleotides: Retention Manipulation via Micellar Solutions and Metal AdditivesAnalytical Chemistry.  59:1021-1027. 1987
1987 Immune recognition of proteins: Conclusions, dilemmas and enigmasBioEssays.  6:112-116. 1987
1987 Immunological self, nonself discriminationScience.  235:865-870. 1987
1987 Reactive sulfhydryl groups of α39, a guanine nucleotide-binding protein from brain. Location and functionJournal of Biological Chemistry.  262:4501-4507. 1987
1987 Site-directed mutagenesis of stahphylococcal nuclease: Role of tyrosine residues in substrate binding and catalysisNucleosides and Nucleotides.  6:331-334. 1987
1987 T cell activation by processed antigen is equally blocked by I‐E and I‐A‐restricted immunodominant peptidesEuropean Journal of Immunology.  17:1605-1609. 1987
1986 Identification of a partial cDNA clone for the C3d/Epstein-Barr virus receptor of human B lymphocytes: Homology with the receptor for fragments C3b and C4b of the third and fourth components of complementProceedings of the National Academy of Sciences.  83:5639-5643. 1986
1986 Identification of a putative second T-cell receptorNature.  322:145-149. 1986
1986 Interaction of peptide antigens and class II major histocompatibility complex antigensNature.  324:260-262. 1986
1986 Molecular cloning and characterization of cDNA encoding the GTP-binding protein α(i) and identification of a related protein, α(h)Proceedings of the National Academy of Sciences.  83:7663-7667. 1986
1986 The T cell repertoire for recognition of a phylogenetically distant protein antigen: Peptide specificity and mhc restriction of staphylococcal nuclease-specific T cell clonesJournal of Experimental Medicine.  164:897-910. 1986
1986 Purification of the B lymphocyte receptor for the C3d fragment of complement and the Epstein-Barr virus by monoclonal antibody affinity chromatography, and assessment of its functional capacitiesJournal of Immunological Methods.  92:79-87. 1986
1986 Antigenic determinants in proteins coincide with surface regions accessible to large probes (antibody domains)Proceedings of the National Academy of Sciences.  83:226-230. 1986
1986 Human tumor cells synthesize an endothelial cell growth factor that is structurally related to basic fibroblast growth factorProceedings of the National Academy of Sciences.  83:2448-2452. 1986
1986 Identification of functional regions on the I-Ab molecule by site-directed mutagenesisProceedings of the National Academy of Sciences.  83:747-751. 1986
1986 Molecular cartography of globular proteins with application to antigenic sitesBiopolymers.  25:863-883. 1986
1985 Expression of murine 21-hydroxylase in mouse adrenal glands and in transfected Y1 adrenocortical tumor cellsProceedings of the National Academy of Sciences.  82:7860-7864. 1985
1985 Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4bProceedings of the National Academy of Sciences.  82:7711-7715. 1985
1985 Purification and amino-terminal protein sequence analysis of the mumps virus fusion proteinVirology.  144:373-383. 1985
1985 Combinational diversity within variable regions bearing the predominant anti-ρ-azophenylarsonate idiotype of strain a miceJournal of Immunology.  134:2740-2747. 1985
1985 Conformation of the eosinophil chemotactic tetrapeptides and analogues in dimethyl sulfoxide. 1H n.m.r. studiesInternational journal of peptide and protein research.  25:337-346. 1985
1985 Molecular studies of the atrial natriuretic factor geneHypertension.  7:31-34. 1985
1985 Rapid purification of the human C3b/C4b receptor (CR1) by monoclonal antibody affinity chromatographyJournal of Immunological Methods.  82:303-313. 1985
1985 Rat epidermal growth factor: complete amino acid sequence: Homology with the correspondence murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activityEuropean Journal of Biochemistry.  153:629-637. 1985
1985 Turns in Peptides and ProteinsAdvances in Protein Chemistry.  37:1-109. 1985
1984 Complete Amino Acid Sequence of the Heavy-Chain Variable Region from an A/J Mouse Antigen-Nonbinding Monoclonal Antibody Bearing the Predominant Arsonate ldiotypeBiochemistry.  23:4726-4732. 1984
1984 Nucleotide sequences of the human and mouse atrial natriuretic factor genesScience.  226:1206-1209. 1984
1984 The structure of rat preproatrial natriuretic factor as defined by a complementary DNA cloneScience.  225:324-326. 1984
1983 Structural Correlates of Idiotypy in the Arsonate SystemAnnals of the New York Academy of Sciences.  418:48-64. 1983
1982 Hollow visceral myopathy. A light- and electron-microscopic studyAmerican Journal of Surgical Pathology.  6:269-275. 1982
1980 Polydispersity of rat mast cell heparin. Implications for proteoglycan assemblyJournal of Biological Chemistry.  255:11753-11758. 1980

Education And Training

  • Master of Science in Medical Office Management / Administration, Tulane University 1998
  • Doctor of Philosophy in Biochemistry, University of Melbourne 1978
  • Doctor of Medicine, University of Missouri System : Columbia 1974
  • Brigham & Women's Hospital, Internship 1981
  • Massachusetts General Hospital, Residency 1983
  • Brigham & Women's Hospital, Residency 1982
  • Brigham & Women's Hospital, Residency 1980
  • Full Name

  • John Smith