Positions

Overview

  • Dr. Cheng completed his medical education at Hunan Medical University in 1985. Later, he continued his graduate training at the Chinese Academy of Medical Sciences & Peking Union Medical College, and Hunan Medical University, where he later became a faculty member. Dr. Cheng served as vice chairman of the Center for Molecular Biology at Hunan Medical University from 1993 to 1996. He completed additional postdoctoral training at the Kimball Research Institute (New York Blood Center) and Emory University School of Medicine. At Emory University, he was promoted to the rank of Instructor and later, Assistant Professor of Pathology and Laboratory Medicine.

    In 2009, he was recruited to the Division of Pulmonary, Allergy & Critical Care Medicine at the University of Alabama at Birmingham at the rank of Associate Professor of Medicine. Dr. Cheng has spent more than two decades in biochemistry, molecular biology, and cell biology research and has discovered nine new human genes in the NADPH oxidase and heme-containing peroxidase families. He holds nine patents issued by the United States Patent and Trademark Office.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2019 Vascular peroxidase 1 is a novel regulator of cardiac fibrosis after myocardial infarctionRedox Biology.  22. 2019
    2018 VPO1 modulates vascular smooth muscle cell phenotypic switch by activating extracellular signal-regulated kinase 1/2 (ERK 1/2) in abdominal aortic aneurysmsJournal of the American Heart Association : Cardiovascular and Cerebrovascular Disease.  7. 2018
    2018 Peroxidasin contributes to lung host defense by direct binding and killing of gram-negative bacteriaPLoS Pathogens.  14. 2018
    2018 Vascular peroxidase 1 mediates hypoxia-induced pulmonary artery smooth muscle cell proliferation, apoptosis resistance and migrationCardiovascular Research.  114:188-199. 2018
    2017 The role of losartan in preventing vascular remodeling in spontaneously hypertensive rats by inhibition of the H2O2/VPO1/HOCl/MMPs pathwayBiochemical and Biophysical Research Communications.  493:855-861. 2017
    2017 Critical role of vascular peroxidase 1 in regulating endothelial nitric oxide synthaseRedox Biology.  12:226-232. 2017
    2017 Involvement of vascular peroxidase 1 in angiotensin II–induced hypertrophy of H9c2 cellsJournal of the American Society of Hypertension.  11:519-529.e1. 2017
    2016 Vascular peroxidase 1 up regulation by angiotensin II attenuates nitric oxide production through increasing asymmetrical dimethylarginine in HUVECsJournal of the American Society of Hypertension.  10:741-751.e3. 2016
    2016 NADPH oxidase 1 is associated with altered host survival and T cell phenotypes after influenza A virus infection in micePLoS ONE.  11. 2016
    2016 VPO1 mediates oxidation of LDL and formation of foam cellsOncotarget.  7:35500-35511. 2016
    2015 The role of vascular peroxidase 1 in ox-LDL-induced vascular smooth muscle cell calcificationAtherosclerosis.  243:357-363. 2015
    2015 Myeloperoxidase Is Increased in Human Cerebral Aneurysms and Increases Formation and Rupture of Cerebral Aneurysms in MiceStroke.  46:1651-1656. 2015
    2014 Negative regulation of NADPH oxidase 4 by Hydrogen peroxide-inducible clone 5 (Hic-5) proteinJournal of Biological Chemistry.  289:18270-18278. 2014
    2014 NADPH oxidases in lung health and diseaseAntioxidants and Redox Signaling.  20:2838-2853. 2014
    2014 Meta-analysis of myeloperoxidase gene polymorphism and coronary artery disease susceptibilityZhongnan Daxue Xuebao. (Yixue Ban) ==.  39:217-231. 2014
    2013 Vascular VPO1 expression is related to the endothelial dysfunction in spontaneously hypertensive ratsBiochemical and Biophysical Research Communications.  439:511-516. 2013
    2013 Fluorofenidone inhibits nicotinamide adeninedinucleotide phosphate oxidase via PI3K/Akt pathway in the pathogenesis of renal interstitial fibrosisNephrology.  18:690-699. 2013
    2013 VPO1 Mediates ApoE Oxidation and Impairs the Clearance of Plasma LipidsPLoS ONE.  8. 2013
    2013 Annexin A1, formyl peptide receptor, and NOX1 orchestrate epithelial repairJournal of Clinical Investigation.  123:443-454. 2013
    2012 Vascular peroxidase 1 catalyzes the formation of hypohalous acids: Characterization of its substrate specificity and enzymatic propertiesFree Radical Biology and Medicine.  53:1954-1959. 2012
    2012 Microbicidal activity of vascular peroxidase 1 in human plasma via generation of hypochlorous acidInfection and Immunity.  80:2528-2537. 2012
    2012 Targeting NOX enzymes in pulmonary fibrosisCellular and Molecular Life Sciences.  69:2365-2371. 2012
    2011 Role of VPO1, a newly identified heme-containing peroxidase, in ox-LDL induced endothelial cell apoptosisFree Radical Biology and Medicine.  51:1492-1500. 2011
    2011 Vascular peroxidase-1 is rapidly secreted, circulates in plasma, and supports dityrosine cross-linking reactionsFree Radical Biology and Medicine.  51:1445-1453. 2011
    2011 Involvement of vascular peroxidase 1 in angiotensin II-induced vascular smooth muscle cell proliferationCardiovascular Research.  91:27-36. 2011
    2008 Identification and characterization of VPO1, a new animal heme-containing peroxidaseFree Radical Biology and Medicine.  45:1682-1694. 2008
    2008 Nox1 is over-expressed in human colon cancers and correlates with activating mutations in K-RasInternational Journal of Cancer.  123:100-107. 2008
    2006 Nox1-dependent reactive oxygen generation is regulated by Rac1Journal of Biological Chemistry.  281:17718-17726. 2006
    2005 Nox1 overexpression potentiates angiotensin II-induced hypertension and vascular smooth muscle hypertrophy in transgenic miceCirculation.  112:2668-2676. 2005
    2005 Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family proteins: mAb 54.1 recognizes recombinant full-length Nox2, Nox3 and the C-terminal domains of Nox1-4 and cross-reacts with GRP 58BBA - Proteins and Proteomics.  1752:186-196. 2005
    2005 Point mutations in the proline-rich region of p22phox are dominant inhibitors of Nox1- and Nox2-dependent reactive oxygen generationJournal of Biological Chemistry.  280:31859-31869. 2005
    2005 Alternative mRNA splice forms of NOXO1: Differential tissue expression and regulation of Nox1 and Nox3Gene.  356:118-126. 2005
    2004 Nox3 regulation by NOXO1, p47phox, and p67phoxJournal of Biological Chemistry.  279:34250-34255. 2004
    2004 The NAD(P)H Oxidase Homolog Nox4 Modulates Insulin-Stimulated Generation of H2O2 and Plays an Integral Role in Insulin Signal TransductionMolecular and Cellular Biology.  24:1844-1854. 2004
    2004 NOXO1, Regulation of Lipid Binding, Localization, and Activation of Nox1 by the Phox Homology (PX) DomainJournal of Biological Chemistry.  279:4737-4742. 2004
    2001 A method of DNA footprinting walking mapShengwu Huaxue Yu Shengwu Wuli Jinzhan.  28:554-555. 2001
    2001 Tyrosine cross-linking of extracellular matrix is catalyzed by Duox, a multidomain oxidase/peroxidase with homology to the phagocyte oxidase subunit gp91phoxJournal of Cell Biology.  154:879-891. 2001
    2001 Homologs of gp91phox: Cloning and tissue expression of Nox3, Nox4, and Nox5Gene.  269:131-140. 2001
    2000 Construction of human stem cell factor's RNA-CRS in quantitative RT-PCRShengwu Huaxue Yu Shengwu Wuli Jinzhan.  27:318. 2000
    2000 Identification of novel regulatory elements of human stem cell factor gene in MCF cellActa Biochimica et Biophysica Sinica -Chinese Edition-.  32:618-619. 2000
    2000 Novel homologs of gp91phoxTrends in Biochemical Sciences.  25:459-461. 2000
    2000 Evans antigen: A new hybrid structure occurring on background of D·· and D-- Rh complexesVox Sanguinis.  78:44-51. 2000
    1999 Molecular basis for Rh(null) syndrome: Identification of three new missense mutations in the Rh50 glycoprotein geneAmerican Journal of Hematology.  62:25-32. 1999
    1999 Molecular basis for Rh(null) syndrome: identification of three new missense mutations in the Rh50 glycoprotein gene.American Journal of Hematology.  62:25-32. 1999
    1999 Rh(mod) syndrome: A family study of the translation-initiator mutation in the Rh50 glycoprotein geneAmerican Journal of Human Genetics.  64:108-117. 1999
    1998 Rh50 glycoprotein gene and RH(null) disease: A silent splice donor is trans to a Gly279→Glu missense mutation in the conserved transmembrane segmentBlood.  92:1776-1784. 1998
    1996 Studies on molecular mechanism of isoniazid resistance in mycobacterium tuberculosisBulletin- Hunan Medical University.  21:501-504. 1996

    Research Overview

  • Dr. Cheng’s research interests also include exploration of the innate host defenses of VPO1 and MPO as well as development of hPx enzymes as antimicrobial agents, particularly for the drug-resistant microbes.

    In the past two decades, Dr. Cheng has identified and characterized five novel members of NADPH oxidases (Nox enzymes), two regulatory proteins of Nox enzymes, and two members of heme-containing peroxidase (hPx enzymes) family (e.g. Nox3, Nox4, Nox5, NOXO1, NOXA1, VPO1 and VPO2). Based on the functional association of the two enzyme families, Dr. Cheng’s research focuses on understanding the synergistic functions of these two enzyme families in maintaining the balance of reactive oxygen species (ROS) in cells, and in pathology, the outcome of ROS imbalance in cardiovascular and respiratory systems. These include the regulatory mechanism of expression and activation of Nox and hPx enzymes.

    Growing evidence indicates that H2O2 functions as a signaling molecule in cells. Recently, Dr. Cheng's group has identified that vascular peroxidase1 (VPO1) is an endogenously negative regulator of H2O2-mediated cellular signaling. Current working hypothesis is that VPO1 mediates diverse physiological and pathological processes via H2O2 on angiogenesis, extracellular matrix formation, cell proliferation, differentiation, and inflammatory responses.

    hPx enzymes catalyze peroxidase reactions and halide oxidation, and produce potent oxidants. VPO1 is highly expressed in the cardiovascular system and lung, and secreted into plasma and bronchoalveolar lavage fluid, leading to the hypothesis that VPO1 plays an important role in the development of atherosclerosis and other inflammatory diseases such as asthma and fibrosis. In addition, the research interests include the involvement of VPO1 in the metabolism of nitric oxide, the formation of di-tyrosine cross-link and the chlorination of proteins.
  • Education And Training

  • Doctor of Philosophy in Molecular Biology, 1996
  • Master of Science in Biochemistry, Biophysics and Molecular Biology, Peking Union Medical College 1990
  • Doctor of Medicine, 1985
  • Full Name

  • Guangjie Cheng