• I am generally interested in discovering and characterizing mechanisms by which cells control gene expression. Much of my career has focused on the regulation of ribosomal RNA synthesis, using an array of biochemical, genetic, and genomic approaches. As an independent academic investigator, I am privileged to be involved in the act of discovery every day. In addition, I take my roles as mentor and educator equally serious. Our goal is to create an environment where we can aggressively ask and answer important questions while training the next generation of scientists to be careful, critical, and content in the pursuit of knowledge.
  • Selected Publications

    Academic Article

    Year Title Altmetric
    2018 NETSeq reveals heterogeneous nucleotide incorporation by RNA polymerase I.Proceedings of the National Academy of Sciences.  115:E11633-E11641. 2018
    2018 The A12.2 Subunit Is an Intrinsic Destabilizer of the RNA Polymerase I Elongation Complex.Biophysical Journal.  114:2507-2515. 2018
    2018 Small-Molecule Targeting of RNA Polymerase I Activates a Conserved Transcription Elongation Checkpoint.Cell Reports.  23:404-414. 2018
    2017 Targeting RNA-Polymerase I in Both Chemosensitive and Chemoresistant Populations in Epithelial Ovarian Cancer.Clinical Cancer Research.  23:6529-6540. 2017
    2017 Quantifying the influence of 5'-RNA modifications on RNA polymerase I activity.Biophysical Chemistry.  230:84-88. 2017
    2017 Multisubunit RNA Polymerase Cleavage Factors Modulate the Kinetics and Energetics of Nucleotide Incorporation: An RNA Polymerase I Case Study.Biochemistry.  56:5654-5662. 2017
    2017 Transcription factors that influence RNA polymerases I and II: To what extent is mechanism of action conserved?BBA - Gene Structure and Expression.  1860:246-255. 2017
    2017 Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics.Methods in Enzymology.  582:415-435. 2017
    2016 The Transcription Factor THO Promotes Transcription Initiation and Elongation by RNA Polymerase I.Journal of Biological Chemistry.  291:3010-3018. 2016
    2016 Direct Characterization of Transcription Elongation by RNA Polymerase I.PLoS ONE.  11:e0159527. 2016
    2015 Transient-State Kinetic Analysis of the RNA Polymerase I Nucleotide Incorporation Mechanism.Biophysical Journal.  109:2382-2393. 2015
    2015 Spt6 Is Essential for rRNA Synthesis by RNA Polymerase I.Molecular and Cellular Biology.  35:2321-2331. 2015
    2015 Functional divergence of eukaryotic RNA polymerases: unique properties of RNA polymerase I suit its cellular role.Gene.  556:19-26. 2015
    2015 A Single Molecule Perspective of Elongation by RNA Polymerase IBiophysical Journal.  108:508a-508a. 2015
    2015 Purification of active RNA polymerase I from yeast.Methods in Molecular Biology.  1276:281-289. 2015
    2014 Hu antigen R (HuR) is a positive regulator of the RNA-binding proteins TDP-43 and FUS/TLS: implications for amyotrophic lateral sclerosis.Journal of Biological Chemistry.  289:31792-31804. 2014
    2014 Using heterogeneity of the patient-derived xenograft model to identify the chemoresistant population in ovarian cancer.Oncotarget.  5:8750-8764. 2014
    2014 A user's guide to the ribosomal DNA in Saccharomyces cerevisiae.Methods in Molecular Biology.  1205:303-328. 2014
    2014 Differences in the composition of the human antibody repertoire by B cell subsets in the blood.Frontiers in Immunology.  5:96. 2014
    2014 Single Molecule Investigation of RNA Polymerase I using Multiplexed Tethered Particle MotionBiophysical Journal.  106:489a-489a. 2014
    2013 Ubp-M serine 552 phosphorylation by cyclin-dependent kinase 1 regulates cell cycle progression.Cell Cycle.  12:3219-3227. 2013
    2013 Divergent contributions of conserved active site residues to transcription by eukaryotic RNA polymerases I and II.Cell Reports.  4:974-984. 2013
    2013 DNA binding by the ribosomal DNA transcription factor rrn3 is essential for ribosomal DNA transcription.Journal of Biological Chemistry.  288:9135-9144. 2013
    2013 Single Molecule Characterization of RNA Polymerase I: Force Free Kinetic MeasurementsBiophysical Journal.  104:585a-585a. 2013
    2013 The SWI/SNF chromatin remodeling complex influences transcription by RNA polymerase I in Saccharomyces cerevisiae.PLoS ONE.  8:e56793. 2013
    2012 RNA polymerase I activity is regulated at multiple steps in the transcription cycle: recent insights into factors that influence transcription elongation.Gene.  493:176-184. 2012
    2012 Efficient transcription by RNA polymerase I using recombinant core factor.Gene.  492:94-99. 2012
    2012 Quantitative analysis of transcription elongation by RNA polymerase I in vitro.Methods in Molecular Biology.  809:579-591. 2012
    2011 The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively.Journal of Biological Chemistry.  286:18816-18824. 2011
    2011 Yeast transcription elongation factor Spt5 associates with RNA polymerase I and RNA polymerase II directly.Journal of Biological Chemistry.  286:18825-18833. 2011
    2010 The RNA polymerase-associated factor 1 complex (Paf1C) directly increases the elongation rate of RNA polymerase I and is required for efficient regulation of rRNA synthesis.Journal of Biological Chemistry.  285:14152-14159. 2010
    2009 Cdc14 inhibits transcription by RNA polymerase I during anaphase.Nature.  458:219-222. 2009
    2009 The Paf1 complex is required for efficient transcription elongation by RNA polymerase I.Proceedings of the National Academy of Sciences.  106:2153-2158. 2009
    2008 Visual analysis of the yeast 5S rRNA gene transcriptome: regulation and role of La protein.Molecular and Cellular Biology.  28:4576-4587. 2008
    2007 Transcription elongation by RNA polymerase I is linked to efficient rRNA processing and ribosome assembly.Molecular Cell.  26:217-229. 2007
    2006 RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing.Proceedings of the National Academy of Sciences.  103:12707-12712. 2006
    2005 Histones are required for transcription of yeast rRNA genes by RNA polymerase I.Proceedings of the National Academy of Sciences.  102:10129-10134. 2005
    2004 RNA polymerase I remains intact without subunit exchange through multiple rounds of transcription in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences.  101:15112-15117. 2004
    2004 DksA: a critical component of the transcription initiation machinery that potentiates the regulation of rRNA promoters by ppGpp and the initiating NTP.Cell.  118:311-322. 2004
    2004 Relationship between growth rate and ATP concentration in Escherichia coli: a bioassay for available cellular ATP.Journal of Biological Chemistry.  279:8262-8268. 2004
    2003 Changes in Escherichia coli rRNA promoter activity correlate with changes in initiating nucleoside triphosphate and guanosine 5' diphosphate 3'-diphosphate concentrations after induction of feedback control of ribosome synthesis.Journal of Bacteriology.  185:6185-6191. 2003
    2003 Changes in the concentrations of guanosine 5'-diphosphate 3'-diphosphate and the initiating nucleoside triphosphate account for inhibition of rRNA transcription in fructose-1,6-diphosphate aldolase (fda) mutants.Journal of Bacteriology.  185:6192-6194. 2003
    2003 Control of rRNA expression by small molecules is dynamic and nonredundant.Molecular Cell.  12:125-134. 2003
    2003 An intersubunit contact stimulating transcription initiation by E coli RNA polymerase: interaction of the alpha C-terminal domain and sigma region 4.Genes and Development.  17:1293-1307. 2003
    2003 Control of rRNA expression in Escherichia coli.Current Opinion in Microbiology.  6:151-156. 2003
    2003 Measuring control of transcription initiation by changing concentrations of nucleotides and their derivatives.Methods in Enzymology.  370:606-617. 2003
    2002 NTP-sensing by rRNA promoters in Escherichia coli is direct.Proceedings of the National Academy of Sciences.  99:8602-8607. 2002
    2001 Generalized transduction in Streptomyces coelicolor.Proceedings of the National Academy of Sciences.  98:6289-6294. 2001

    Teaching Overview

  • I teach courses aimed primarily toward graduate students and professional students. My overall approach to education takes into account the fact that these students are adults who are well-trained, but who desire advanced education in research. I create courses where students can learn advanced topics in gene expression, RNA biology, and grant writing. In those courses, the environment is designed to feed a students curiosity and satisfy the desire for knowledge and personal improvement. I do not use traditional test-based approaches or negative reinforcement as motivation. To me, the students and I are grown-ups. Let's learn together as such.
  • Education And Training

  • Doctor of Philosophy in Microbiology, University of Wisconsin System : Madison
  • Full Name

  • David Schneider
  • Blazerid

  • dschneid