Positions

Selected Publications

Academic Article

Year Title Altmetric
2018 Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1BBA - Biomembranes.  1860:1193-1204. 2018
2018 Ligand binding to a remote site thermodynamically corrects the F508del mutation in the human cystic fibrosis transmembrane conductance regulatorJournal of Biological Chemistry.  293:17685-17704. 2018
2017 Stabilization of a nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator yields insight into disease-causing mutationsJournal of Biological Chemistry.  292:14147-14164. 2017
2017 Direct binding of the Corrector VX-809 to Human CFTR NBD1: Evidence of an Allosteric coupling between the Binding site and the NBD1:CL4 Interface sMolecular Pharmacology.  92:124-135. 2017
2017 Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and functionBBA - Biomembranes.  1859:48-60. 2017
2016 A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in DetergentsMethods in Enzymology.  567:319-358. 2016
2015 Restoration of NBD1 thermal stability is necessary and sufficient to correct Δf508 CFTR folding and assemblyJournal of Molecular Biology.  427:106-120. 2015
2014 Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domainsProtein Science.  23:769-789. 2014
2012 Conformational changes relevant to channel activity and folding within the first nucleotide binding domain of the cystic fibrosis transmembrane conductance regulatorJournal of Biological Chemistry.  287:28480-28494. 2012
2011 A gene optimization strategy that enhances production of fully functional P-Glycoprotein in Pichia pastorisPLoS ONE.  6. 2011
2010 Integrated biophysical studies implicate partial unfolding of NBD1 of CFTR in the molecular pathogenesis of F508del cystic fibrosisProtein Science.  19:1932-1947. 2010
2010 Thermal unfolding studies show the disease causing F508del mutation in CFTR thermodynamically destabilizes nucleotide-binding domain 1Protein Science.  19:1917-1931. 2010
2010 Structures of a minimal human CFTR first nucleotide-binding domain as a monomer, head-to-tail homodimer, and pathogenic mutantProtein Engineering -Oxford-.  23:375-384. 2010
2009 SAR studies for a new class of antibacterial NAD biosynthesis inhibitorsJournal of Combinatorial Chemistry.  11:617-625. 2009
2009 Virtual screening to identify lead inhibitors for bacterial NAD synthetase (NADs)Bioorganic and Medicinal Chemistry Letters.  19:2001-2005. 2009
2008 Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracisActa Crystallographica Section F: Structural Biology and Crystallization Communications.  64:893-898. 2008
2007 Structural adaptation of an interacting non-native C-terminal helical extension revealed in the crystal structure of NAD+ synthetase from Bacillus anthracisActa Crystallographica Section D: Biological Crystallography.  63:891-905. 2007
2007 Antibacterial nicotinamide adenine dinucleotide synthetase inhibitors: Amide- and ether-linked tethered dimers with α-amino acid end groupsJournal of Medicinal Chemistry.  50:2612-2621. 2007
2007 His-tag impact on structureActa Crystallographica Section D: Biological Crystallography.  63:295-301. 2007
2005 Förster resonance energy transfer measurements are consistent with a helical bundle model for lipid-free apolipoprotein A-IBiochemistry.  44:16413-16425. 2005
2005 Engineering mouse apolipoprotein A-I into a monomeric, active protein useful for structural determinationBiochemistry.  44:14907-14919. 2005
2005 Tethered dimer inhibitors of NAD synthetase: Parallel synthesis of an aryl-substituted SAR libraryJournal of Combinatorial Chemistry.  7:898-904. 2005
2004 Role of inhibitor aliphatic chain in the thermodynamics of inhibitor binding to Escherichia coli enoyl-ACP reductase and the Phe203Leu mutant: A proposed mechanism for drug resistanceBiochemistry.  43:13380-13389. 2004
2004 Dimethyl sulfoxide at 2.5% (v/v) alters the structural cooperativity and unfolding mechanism of dimeric bacterial NAD + synthetaseProtein Science.  13:830-841. 2004
2003 Deletion of DnaK's lid strengthens binding to the nucleotide exchange factor, GrpE: A kinetic and thermodynamic analysisBiochemistry.  42:9028-9040. 2003
2003 Tethered dimers as NAD synthetase inhibitors with antibacterial activityJournal of Medicinal Chemistry.  46:3371-3381. 2003
2002 NH3-dependent NAD+ synthetase from Bacillus subtilis at 1 Å resolutionActa Crystallographica Section D: Biological Crystallography.  58:1138-1146. 2002
2001 Kinetic and calorimetric evidence for two distinct scaffolding protein binding populations within the bacteriophage P22 procapsidBiochemistry.  40:8962-8970. 2001
2001 Stabilization of active-site loops in NH3-dependent NAD+ synthetase from Bacillus subtilisActa Crystallographica Section D: Biological Crystallography.  57:806-812. 2001
2001 Structural models of human apolipoprotein A-I: A critical analysis and reviewBiochimica et Biophysica Acta Molecular and Cell Biology of Lipids.  1531:4-46. 2001
2000 Stereoselective binding of an enantiomeric pair of stromelysin-1 inhibitors caused by conformational entropy factorsBioorganic and Medicinal Chemistry Letters.  10:2427-2430. 2000
2000 Down-regulation of cell surface receptors is modulated by polar residues within the transmembrane domainCell regulation.  11:2643-2655. 2000
1999 Human apolipoprotein A-I: Structure determination and analysis of unusual diffraction characteristicsActa Crystallographica Section D: Biological Crystallography.  55:2013-2021. 1999
1999 Analysis of the binding of hydroxamic acid and carboxylic acid inhibitors to the stromelysin-1 (matrix metalloproteinase-3) catalytic domain by isothermal titration calorimetryBiochemistry.  38:13592-13601. 1999
1999 Crystallization of truncated human apolipoprotein A-I in a novel conformationActa Crystallographica Section D: Biological Crystallography.  55:1578-1583. 1999
1999 The structure of human lipoprotein A-I. Evidence for the 'belt' modelJournal of Biological Chemistry.  274:14541-14544. 1999
1998 The lipid-free structure of apolipoprotein A-I: Effects of amino- terminal deletionsBiochemistry.  37:11714-11725. 1998
1998 Structural analysis of apolipoprotein A-I: Effects of amino- and carboxy-terminal deletions on the lipid-free structureBiochemistry.  37:945-955. 1998
1997 Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformationProceedings of the National Academy of Sciences.  94:12291-12296. 1997
1997 Structural analysis of apolipoprotein A-I: Limited proteolysis of methionine-reduced and -oxidized lipid-free and lipid-bound human apo A-IBiochemistry.  36:7615-7624. 1997
1997 Truncation of the amine terminus of human apolipoprotein A-I substantially alters only the lipid-free conformationBiochemistry.  36:288-300. 1997
1996 Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 bindingProceedings of the National Academy of Sciences.  93:15030-15035. 1996
1996 Storage stability of the solution formulation of sCD4 determined by DSC in comparison with two functional assaysJournal of Thermal Analysis and Calorimetry.  47:1597-1609. 1996
1995 Structural models of human apolipoprotein A-IBBA - Lipids and Lipid Metabolism.  1256:103-129. 1995
1995 Interdomain Communication of T-Cell CD4 Studied by Absorbance and Fluorescence Difference Spectroscopy Measurements of Urea-Induced UnfoldingBiochemistry.  34:6464-6474. 1995
1994 The amphipathic α helix: A multifunctional structural motif in plasma apolipoproteinsAdvances in Protein Chemistry.  45:303-369. 1994
1993 Intracellular transport and virion incorporation of vpx requires interaction with other virus type-specific componentsVirology.  193:222-233. 1993
1992 Analysis of the Two-State Behavior of the Thermal Unfolding of Serum Retinol Binding Protein Containing a Single Retinol LigandBiochemistry.  31:5560-5567. 1992
1992 The amphipathic helix in the exchangeable apolipoproteins: A review of secondary structure and functionJournal of Lipid Research.  33:141-166. 1992
1992 Thermal stabilities of mutant Escherichia coli tryptophan synthase α subunitsArchives of Biochemistry and Biophysics.  292:34-41. 1992
1991 Role of amphipathic helixes in HDL structure/functionAdvances in human genetics.  285:131-140. 1991
1990 Amphipathic helix motif: Classes and propertiesProteins.  8:103-117. 1990
1990 Use of synthetic peptide analogues to localize lecithin:cholesterol acyltransferase activating domain in apolipoprotein A-IArteriosclerosis (Dallas, Tex.).  10:95-105. 1990
1989 Characterization of apoA-I-containing lipoprotein subpopulations secreted by HepG2 cellsJournal of Lipid Research.  30:1429-1436. 1989
1989 Structure and thermal stability of monomeric bacteriorhodopsin in mixed pospholipid/detergent micellesProteins.  5:38-46. 1989
1987 Characterization of high density lipoprotein subspecies: Structural studies by single vertical spin ultracentrifugation and immunoaffinity chromatographyJournal of Lipid Research.  28:913-929. 1987
1987 pH Dependence of Bacteriorhodopsin Thermal UnfoldingBiochemistry.  26:7431-7438. 1987
1985 Studies of synthetic peptide analogs of the amphiphatic helix. Correlation of structure with functionJournal of Biological Chemistry.  260:10256-10262. 1985
1985 Studies of synthetic peptide analogs of the amphiphatic helix. Structure of complexes with dimyristoyl phosphatidylcholineJournal of Biological Chemistry.  260:10248-10255. 1985
1984 Structural Studies of Apolipoprotein A-I/Phosphatidylcholine Recombinants by High-Field Proton NMR, Nondenaturing Gradient Gel Electrophoresis, and Electron MicroscopyBiochemistry.  23:359-367. 1984
1983 Studies of synthetic peptide analogs of the amphipathic helix. Competitive displacement of exchangeable apolipoproteins from native lipoproteinsJournal of Biological Chemistry.  258:2290-2295. 1983
1982 Structural domains of vesicular stomatitis virus. A study by differential scanning calorimetry, thermal gel analysis, and thermal electron microscopy.Journal of Biological Chemistry.  257:12-15. 1982
1982 Dynamics of Apolipoprotein-Phospholipid InteractionsBiophysical Journal.  37:172-173. 1982
1982 Minimal Size Phosphatidylcholine Vesicles: Effects of Radius of Curvature on Head Group Packing and ConformationBiochemistry.  21:4569-4575. 1982
1982 Structural Domains in Vesicular Stomatitis Virus Membrane as Studied by Differential Scanning CalorimetryBiophysical Journal.  37:25-26. 1982

Education And Training

  • Doctor of Philosophy in Medicinal and Pharmaceutical Chemistry, University of Kansas 1979
  • Full Name

  • Christie Brouillette