Positions

Selected Publications

Academic Article

Year Title Altmetric
2019 DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substratesNature Communications.  10. 2019
2019 Pharmacologic inhibition of LIMK1 provides dendritic spine resilience against β-amyloidScience Signaling.  12. 2019
2019 The desmosome is a mesoscale lipid raft–like membrane domain 2019
2018 Cutting edge: Intracellular IFN-β and distinct type i IFN expression patterns in circulating systemic lupus erythematosus B cellsJournal of Immunology.  201:2203-2208. 2018
2018 Mapping the 3D orientation of piconewton integrin traction forcesNature methods.  15:115-118. 2018
2018 Bridging the gap: Super-resolution microscopy of epithelial cell junctionsTissue Barriers.  6. 2018
2017 Desmoglein 3 Order and Dynamics in Desmosomes Determined by Fluorescence Polarization MicroscopyBiophysical Journal.  113:2519-2529. 2017
2017 High-resolution imaging of muscle attachment structures in Caenorhabditis elegansCytoskeleton.  74:426-442. 2017
2016 Regulation of claudin/zonula occludens-1 complexes by hetero-claudin interactionsNature Communications.  7. 2016
2016 Nanoscale optomechanical actuators for controlling mechanotransduction in living cellsNature methods.  13:143-146. 2016
2016 Molecular organization of the desmosome as revealed by direct stochastic optical reconstruction microscopyJournal of Cell Science.  129:2897-2904. 2016
2016 Super-resolution microscopy reveals altered desmosomal protein organization in tissue from patients with pemphigus vulgarisJournal of Investigative Dermatology.  136:59-66. 2016
2016 The sodium chloride cotransporter (NCC) and epithelial sodium channel (ENaC) associateBiochemical Journal.  473:3237-3252. 2016
2015 ROCK1 and ROCK2 inhibition alters dendritic spine morphology in hippocampal neurons.Cellular Logistics.  5:e1133266. 2015
2015 Real-time fluorescence imaging with 20 nm axial resolutionNature Communications.  6. 2015
2014 The ARL2 GTPase is required for mitochondrial morphology, motility, and maintenance of ATP levelsPLoS ONE.  9. 2014
2014 Polarization-controlled TIRFM with focal drift and spatial field intensity correctionBiophysical Journal.  106:1008-1019. 2014
2014 Desmosome assembly and disassembly are membrane raft-dependentPLoS ONE.  9. 2014
2013 Pharmacologic inhibition of ROCK2 suppresses amyloid-β production in an Alzheimer's disease mouse model 2013
2013 The Chlamydomonas mutant pf27 reveals novel features of ciliary radial spoke assemblyCytoskeleton.  70:804-818. 2013
2013 Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis 2013
2013 Conserved spatial organization of FG domains in the nuclear pore complexBiophysical Journal.  104:37-50. 2013
2012 Signaling network crosstalk in human pluripotent cells: A Smad2/3-regulated switch that controls the balance between self-renewal and differentiationCell Stem Cell.  10:312-326. 2012
2012 Domain topology of nucleoporin Nup98 within the nuclear pore complex 2012
2011 Imaging single endocytic events reveals diversity in clathrin, dynamin and vesicle dynamicsTraffic.  12:1394-1406. 2011
2011 Mapping the orientation of nuclear pore proteins in living cells with polarized fluorescence microscopy 2011
2010 Imaging with total internal reflection fluorescence microscopy for the cell biologistJournal of Cell Science.  123:3621-3628. 2010
2010 Fluorescence anisotropy reveals order and disorder of protein domains in the nuclear pore complexBiophysical Journal.  99:1706-1717. 2010
2009 Spatial and temporal dynamics of mitochondrial membrane permeability waves during apoptosisBiophysical Journal.  97:2222-2231. 2009
2006 Effective elimination of laser interference fringing in fluorescence microscopy by spinning azimuthal incidence angleMicroscopy Research and Technique.  69:642-647. 2006
2006 Direct measurement of the evanescent field profile produced by objective-based total internal reflection fluorescenceJournal of Biomedical Optics.  11. 2006
2005 Fluorescence emission patterns near glass and metal-coated surfaces investigated with back focal plane imagingJournal of Biomedical Optics.  10. 2005
2004 Polarized fluorescence resonance energy transfer microscopy.Biophysical Journal.  87:2787-2797. 2004
1998 A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitution 1998

Chapter

Year Title Altmetric
2015 Förster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions.  329-339. 2015

Research Overview

  • The Mattheyses laboratory is interested in how cells interact with each other and their environment, and how the spatial-temporal dynamics and regulation of this communication directly impacts cellular homeostasis and function. We develop and apply innovative fluorescence microscopy techniques to elucidate the dynamics, forces, and organization of proteins within macromolecular assemblies central to cellular communication. Our three main areas of interest are: the organization and regulation of desmosomes, mechano-transduction and molecular forces in cell adhesion, and the spatio-temporal dynamics of endocytosis. Characteristics of proteins in the cellular environment – localization dynamics, higher-order organization and assembly, and mechanical tension – can alter function, yet the number of tools that can dissect the native physical environment of proteins lags behind that of biochemical analyses. Our research combines sophisticated imaging including super-resolution fluorescence microscopy (SIM and STORM), total internal reflection fluorescence (TIRF), fluorescence polarization, and microscopy technique development with primary and continuous cell culture models, molecular biology, theoretical modeling, and image analysis. Our goal is to gain a mechanistic understanding of the dynamics and function of macromolecular complexes in cellular communication and providing insight into the cellular basis for human health and disease.
  • Full Name

  • Alexa Mattheyses